We narrowed to 18,792 results for: tec

1. PlayBack-Ef1aKpn1

   Plasmid

   #203307

   Purpose

   For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. BCJJ125

   Plasmid

   #117515

   Purpose

   Cas9_3 (with intron) Golden Gate Level 0

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BsaI:AATG:Cas9_3(intron):GCTT:BsaI
   

   Use

   CRISPR

   Tags

   2xFLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUSE-RapR-Src-myc

   Plasmid

   #25933

   Depositor

   Klaus Hahn

   Article

   Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

   Insert

   RapR-Src (Src Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G03 PC

   Plasmid

   #67132

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   PC (PC Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

5. MOBE3

   Plasmid

   #219943

   Purpose

   Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-SL3_PT2A_apt-ABE8e
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSIBR4-Cre

   Plasmid

   #219435

   Purpose

   Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

   Depositor

   Sheng Yang

   Article

   Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

   Insert

   intron 4-Cre
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EMM65

   Plasmid

   #49042

   Purpose

   Backbone contains SHD/C 0.5 Domain TALE N-terminal: TAL C-terminus: LSD1: Isoform 2

   Depositor

   Bradley Bernstein, Eric Mendenhall

   Article

   Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

   Type

   Empty backbone

   Use

   Tale lsd1 expression vector

   Tags

   LSD1

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha

   Available since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBad33_ISEc41_reRNA

   Plasmid

   #205341

   Purpose

   Bacterial expression of ISEc41 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISEc41 TnpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBad33_ISEc46_reRNA

   Plasmid

   #205343

   Purpose

   Bacterial expression of ISEc46 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   ISEc46 TnpB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBad33_ISNsp2_reRNA

    Plasmid

    #205350

    Purpose

    Bacterial expression of ISNsp2 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISNsp2 TnpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfB2196

    Plasmid

    #67549

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. GGE146

    Plasmid

    #120742

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_4UASxpr-TEF)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_4UASxpr-TEF
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCOLA-T7-WspR:D70E

    Plasmid

    #79164

    Purpose

    Expresses constitutively active WspR D70E under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D70E

    Promoter

    T7

    Available since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRBBm59

    Plasmid

    #48115

    Purpose

    Shuttle vector E. coli/B.meg.

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

    Insert

    promoter sacB - gfp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. NLS-LgBiT-CaM

    Plasmid

    #183401

    Purpose

    Expresses nuclear-localized LgBiT-CaM

    Depositor

    Alice Ting

    Article

    Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

    Insert

    LgBiT-CaM
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PCS2+ Cas9

    Plasmid

    #122948

    Purpose

    For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 438-GST

    Plasmid

    #97092

    Purpose

    GST-tev-yORF

    Depositor

    Chris Jeans

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    GST tag and TEV cleavage site

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHG4

    Plasmid

    #188252

    Purpose

    Integrating plasmid contains the TG1 int/attP locus and the apramycin-resistance gene (aac(3)IV) for selection

    Depositor

    Maggie Smith, Hong Gao

    Article

    Gao et al BMC Biotechnol. 2019 Jun 4;19(1):32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    act1p/actII-orf4

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBR6k-GFPuv

    Plasmid

    #106384

    Purpose

    IPTG-inducible LlacO-1 promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. GGE104

    Plasmid

    #120744

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Inducible promoter for gene expression in position1 (P1_EYK300-3AB)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P1_EYK300-3AB
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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