We narrowed to 43,146 results for: cha

1. MXS_linker

   Plasmid

   #62431

   Purpose

   MXS_chaining vector with linker

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   Linker 12AA Gly-/Ser-rich
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. MXS_MCS4

   Plasmid

   #62398

   Purpose

   MXS_chaining vector with MCS4

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   MluI-XhoI-PvuII-BglII-SalI MCS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGLx2-set1-H1017K

   Plasmid

   #53258

   Purpose

   GAL driven LexA-tagged SET1-catalytically dead (H1017K)

   Depositor

   Jean Cook

   Article

   Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

   Insert

   SET1
   

   Use

   Tags

   LexA

   Expression

   Yeast

   Mutation

   H1017K mutation - catalytically dead

   Promoter

   GAL1/10

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBACgus N-His/GST/LRRK2 1867-2141

   Plasmid

   #40404

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   GST-Tev and HisTev

   Expression

   Mutation

   WT aa1867-2141

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pBACgus N-His/GST/LRRK2 1867-2161

   Plasmid

   #40405

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   GST-Tev and HisTev

   Expression

   Mutation

   WT aa1867-2161

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pBACgus4x/cdc37/LRRK2 1867-2161_C2024S/C2025S

   Plasmid

   #40372

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   HisTev

   Expression

   Mutation

   aa1867-2161, C2024S/C2025S

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pBACgus_Sumostar/LRRK2 1867-2186_C2024S/C2025S

   Plasmid

   #40388

   Depositor

   Michael J Fox Foundation MJFF

   Inserts

   SUMO
   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   6xHis and HisTEV

   Expression

   Mutation

   aa1867-2186, C2024S/C2025S

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. NHA-Delta 12am

   Plasmid

   #36935

   Depositor

   Edward Chan

   Article

   Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

   Insert

   TNRC6A Delta 12am (TNRC6A Human)
   

   Use

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   aa 896-1045 only; all GW or WG changed to AA

   Promoter

   Available since

   May 30, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Tpa1(21-644)

   Plasmid

   #23140

   Depositor

   SeWon Suh

   Article

   Kim et al Nucleic Acids Res. 2009 Dec 29. ():.

   Insert

   Tpa1 (TPA1 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-20

   Promoter

   Available since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pWZ414-F48

    Plasmid

    #23072

    Depositor

    Sharon Dent

    Article

    Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

    Insert

    yeast Histone H3-2 and Histone H4-2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Histone H3 changed Lysine 14 to Glutamine, H3 K14…

    Promoter

    Available since

    March 3, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pWZ414-F50

    Plasmid

    #23074

    Depositor

    Sharon Dent

    Article

    Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

    Insert

    yeast Histone H3-2 and Histone H4-2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Histone H3 changed Lysine 14 to Arginine, H3 K14R…

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pWZ414-F54

    Plasmid

    #23078

    Depositor

    Sharon Dent

    Article

    Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

    Insert

    yeast Histone H3-2 and Histone H4-2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Histone H3 changed Lysine 9 to Glutamine, H3 K9Q …

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pRS416 Gal-RNQ1 L94A

    Plasmid

    #18691

    Depositor

    Susan Lindquist

    Article

    Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

    Insert

    Rnq1 L94A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 94 mutated to Alanine (L94A)

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pJRL2 GAL1(TATA-A1T6)pr VYFP (EB#1573)

    Plasmid

    #14849

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
    

    Use

    Tags

    VYFP

    Expression

    Yeast

    Mutation

    TATA-A1T6. TATA box mutated from TATATAAG to AATA…

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pJRL2 GAL1(TATA-A1T6)pr ECFP (EB#1574)

    Plasmid

    #14850

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    GAL1 promoter TATA-A1T6 (GAL1 Budding Yeast)
    

    Use

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    TATA-A1T6. TATA box mutated from TATATAAG to AATA…

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

16. Ypd1p-pET22b

    Plasmid

    #12655

    Depositor

    SeWon Suh

    Article

    Song et al J Mol Biol. 1999 Nov 5. 293(4):753-61.

    Insert

    YPD1 protein (YPD1 Budding Yeast)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

17. WT Ecm2

    Plasmid

    #169922

    Purpose

    Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAsp-AMA-gpdA-ptr

    Plasmid

    #191016

    Purpose

    CRISPR vector for cloning sgDNAs to target genes of several Aspergillus species (especially section Flavi)

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Microbiol Spectr. 2023 Feb 14;11(1):e046

    Type

    Empty backbone

    Use

    Unspecified; Aspergillus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ZJOM151

    Plasmid

    #133643

    Purpose

    Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX4-GFP (COX4 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ZJOM18

    Plasmid

    #133631

    Purpose

    Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-GFP (NAB2 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mPiezo1-IRES-eGFP

    Plasmid

    #80925

    Purpose

    expresses mouse Piezo1 with GFP

    Depositor

    Ardem Patapoutian

    Article

    Coste et al Science. 2010 Oct 1;330(6000):55-60. doi

    Insert

    mouse Piezo1 CDS (Piezo1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BFP

    Plasmid

    #201383

    Purpose

    Control nucleocytoplasmic transport probe

    Depositor

    Pere Roca-Cusachs

    Article

    Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

    Insert

    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TPC2-G-GECO1.2

    Plasmid

    #207141

    Purpose

    Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPC2N2 (TPCN2 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TPC1-G-GECO1.2

    Plasmid

    #207143

    Purpose

    Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel

    Depositor

    Antony Galione

    Article

    Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

    Insert

    TPCN1 (TPCN1 Human)
    

    Use

    Tags

    G-GECO1.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. NET47 pEGFP-N2 (593)

    Plasmid

    #61986

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET47 (TM7SF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pBbA5c-MevT-MBIS

    Plasmid

    #35150

    Depositor

    Taek Soon Lee, Jay Keasling

    Article

    Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

    Insert

    AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacUV5,

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NylCK-TS_pET28a

    Plasmid

    #215423

    Purpose

    Expression construct for NylCK-TS gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCK-TS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S111G/A137L

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFA6a-3HA-His3MX6

    Plasmid

    #41600

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Expression

    Mutation

    Promoter

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NylCK_pET28a

    Plasmid

    #215422

    Purpose

    Expression construct for NylCK gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCK
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MXS_CMV::ZeoR-bGHpA

    Plasmid

    #62441

    Purpose

    MXS_chaining vector with CMV::ZeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Zeocin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. tubP-FRT>GAL80-6-FRT>

    Plasmid

    #63173

    Purpose

    ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "Flp-Out" GAL80
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. AAV-syn-ChR2-EYFP-Kv

    Plasmid

    #89256

    Purpose

    Channelrhodopsin localized to neuronal soma via a Kv2.1 targeting motif

    Depositor

    McLean Bolton

    Article

    Baker et al Elife. 2016 Aug 15;5. pii: e14193. doi:

    Insert

    Channelrhodopsin
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    synapsin

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMP11

    Plasmid

    #215559

    Purpose

    pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Inserts

    Cas9
    gRNA targeting pBR322 origin of replication
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23105 and tet

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRG713_lexO-HO_LexA-TF_URA3MX

    Plasmid

    #154821

    Purpose

    Inducible expression of HO endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    HO endonuclease (HO Budding Yeast)
    LexA-ER-B112
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMH1105

    Plasmid

    #131864

    Purpose

    Produces holo-PhyB-AviTag in E. coli

    Depositor

    Wilfried Weber

    Article

    Horner et al Bio Protoc. 2020 Mar 5;10(5):e3541. doi:

    Inserts

    PhyB(1-651)-AviTag-His6
    HO1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter-1 and T7 promoter-2

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pZac2.1 gfaABC1D-eGFP

    Plasmid

    #176861

    Purpose

    Expresses cytosolic eGFP in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. AfNitB_pET29b

    Plasmid

    #215401

    Purpose

    Expression construct for AfNitB gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    AfNitB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3 MYC-Sumo3Q89P

    Plasmid

    #48963

    Purpose

    Expresses MYC-Sumo3Q90P mutant in mammalian cells

    Depositor

    Guy Salvesen

    Article

    Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

    Insert

    SUMO3 (SUMO3 Human)
    

    Use

    Tags

    MYC (EQKLISEED)

    Expression

    Mammalian

    Mutation

    Mutated Q89P

    Promoter

    T7

    Available since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

39. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NylCA_pET28a

    Plasmid

    #215418

    Purpose

    Expression construct for NylCA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylCA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge