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Showing: 2401 - 2420 of 16949 results
  1. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Mutation
    Promoter
    TEF1p
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  2. pBP Omomer

    Plasmid
    #113169
    Purpose
    Retroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.
    Depositor
    Insert
    Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    E57T, E64I, R70Q, R71N
    Promoter
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pcDNA3_erGAP3

    Plasmid
    #78118
    Purpose
    Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum
    Insert
    erGAP3
    Use
    Tags
    KDEL and calreticulin signal peptide
    Expression
    Mammalian
    Mutation
    S175G, D180Y, I167V and L15Q in GFP moiety and D…
    Promoter
    CMV
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pCPP5912

    Plasmid
    #128703
    Purpose
    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone
    Depositor
    Insert
    shcE-avrE1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Three synonymous mutations Ala 362 (GCT-GCC), Ala…
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. Epac-S-H90

    Plasmid
    #170340
    Purpose
    CFP(UST)_EPACdDEPCD_cp173Venus(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Insert
    CFP(UST)_EPACdDEPCD_cp173Venus(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid
    #140163
    Purpose
    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker
    Depositor
    Insert
    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Synthetic, Human)
    Use
    Tags
    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pssGFP-hCRT

    Plasmid
    #80022
    Purpose
    Mammalian expression of signal peptide-EGFP-humanCRT
    Depositor
    Insert
    CRT (CALR Human)
    Use
    Tags
    EGFP and signal peptide
    Expression
    Mammalian
    Mutation
    signal peptide 17aa added before GFP-CRT, will ta…
    Promoter
    CMV
    Available since
    July 27, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2401 - 2420 of 16949 results