We narrowed to 6,138 results for: WUS;

1. pCFJ150-Cas9(dpiRNA)

   Plasmid

   #107940

   Purpose

   eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   Cas9(dpiRNA)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eft-3

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. p21-pRc/CMV

   Plasmid

   #20814

   Depositor

   William Kaelin

   Article

   Adams et al Mol Cell Biol. 1996 Dec . 16(12):6623-33

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   Available since

   April 6, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLN540 (FuGW-G8p-IL12-P2A-CCL21-1Pe)

   Plasmid

   #105201

   Purpose

   Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 and CCL21 transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G8p-IL12-P2A-CCL21-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28a_Pro-IL-36β

   Plasmid

   #214344

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL36B (IL36B Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTorPE-G-GECO1.2

   Plasmid

   #32468

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.2
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

   Expression

   Bacterial

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-TRE-DIO-mRuby2

   Plasmid

   #99117

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   DIO-mRuby2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQ292

   Plasmid

   #129672

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b
   

   Use

   CRISPR; Gateway compatible aacas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   AaCas12b is rice codon optimized

   Promoter

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEAK-cKal7

   Plasmid

   #145801

   Purpose

   expresses Kalirin-7 with the c-front in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

   Insert

   cKalirin-7 (Kalrn Rat)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   EIF2a

   Available since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEAK-bKal7

   Plasmid

   #145800

   Purpose

   expresses Kalirin-7 with the b-front in mammalian cells

   Depositor

   Betty Eipper, Richard Mains

   Article

   Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

   Insert

   bKalirin-7 (Kalrn Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EIF2a

   Available since

   June 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lck-mCherry-RGS

    Plasmid

    #112935

    Purpose

    Plasmid encodes a membrane located inhibitor of Ga12/13 signaling (RGS domain from p115)

    Depositor

    Dorus Gadella

    Article

    Reinhard et al Mol Biol Cell. 2017 Nov 7;28(23):3371-33

    Insert

    p115RhoGEF-RGS domain
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEmerald-H2B-C-10

    Plasmid

    #54112

    Purpose

    Localization: Nucleus/Histones, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. AV4-EF1-NMNAT2

    Plasmid

    #124970

    Purpose

    Express NMNAT2

    Depositor

    Kazuhiro Oka

    Article

    Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

    Insert

    NMNAT2, GFP (NMNAT2 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-N1-8x

    Plasmid

    #172284

    Purpose

    Expressing EGFP mRNA fused with 8 tandem repeats of a 50-base sequence

    Depositor

    Antony K Chen

    Article

    Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

    Insert

    EGFP-N1-8x
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MTK4_001

    Plasmid

    #123810

    Purpose

    Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Bgh pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MTK234_032

    Plasmid

    #123995

    Purpose

    Encodes the spCas9 sgRNA1 hAAVS1 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    sgRNA1 -hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-SV40-Galpha-i1 ONE-GO

    Plasmid

    #204328

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    SV40 Galpha-i1 ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSIN-Ski-gRNA2

    Plasmid

    #180369

    Purpose

    targeting mouse Ski gene

    Depositor

    Dan Littman

    Article

    Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

    Insert

    Ski targeting gRNA (Ski Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-SMVP-Cas9N-U6-gRNA TdL

    Plasmid

    #80938

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3XFLAG-TAK1-K63A

    Plasmid

    #43933

    Depositor

    Bryant Darnay

    Article

    Besse et al J Biol Chem. 2007 Feb 9;282(6):3918-28.

    Insert

    Transforming growth factor-beta-activated kinase 1 (Map3k7 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    K63A

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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