We narrowed to 165,918 results for: addgene

1. M4755 KanMX::LEU2 Disruptor Converter

   Plasmid

   #51685

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to LEU2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   LEU2 (LEU2 Budding Yeast)
   

   Use

   Yeast marker swap

   Expression

   Yeast

   Available Since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Id2 Ngfr

   Plasmid

   #80131

   Purpose

   overexpression of ID2

   Depositor

   Ellen Rothenberg

   Article

   Del Real et al Development. 2013 Mar;140(6):1207-19. do

   Insert

   Id2 (Id2 Mouse)
   

   Use

   Retroviral

   Tags

   IRES-Ngfr

   Expression

   Mammalian

   Available Since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. ZAK (ZAKA-c057)

   Plasmid

   #98225

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ZAKA (MAP3K20 Human)
   

   Use

   Baculovirus

   Tags

   His-6-TEV

   Mutation

   pfam00069:Pkinase 16-257

   Promoter

   polyhedrin

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. ULK1 KO sgRNA

   Plasmid

   #207561

   Purpose

   pX330 expressing Cas9 and a sgRNA targeting the ULK1 locus

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   CCCGCCTGCGCCATGGAGCC
   

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEVY-GA

   Plasmid

   #51227

   Purpose

   bi-directional expression vector for yeast, galactose inducible, ADE2 selection

   Depositor

   Charles Miller

   Article

   Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GAL1,10

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR_eGFP_191

   Plasmid

   #111821

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 171-191
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pI6-MTU-DO-URA

   Plasmid

   #160030

   Purpose

   Integrative vector for K. marxianus, targeting the ARO3 locus. Uracil selection. Low integration efficiency in an NHEJ-deficient background.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Expression

   Yeast

   Promoter

   n/a

   Available Since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG306-GPD-AVT1 chr I

   Plasmid

   #41898

   Purpose

   Yeast expression of AVT1.

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Insert

   AVT1 (AVT1 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe NEO Venus

   Plasmid

   #98396

   Purpose

   Retroviral vector for constitutive Venus expression

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   Venus
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVU-tTR-KRAB

    Plasmid

    #11645

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, no tetO
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEpic

    Plasmid

    #84372

    Purpose

    3rd-generation lentiviral destination vector for gateway cloning with puromycin resistance.

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-Arch-EEQ

    Plasmid

    #45188

    Purpose

    Arch voltage indicator with double mutations D95Q-D106E (Arch-EEQ), with faster kinetics and greater fluorescence dynamic range than Arch-D95N

    Depositor

    Mark Schnitzer

    Article

    Gong et al PLoS One. 2013 Jun 19;8(6):e66959.

    Insert

    enhanced Archaerhodopsin
    

    Use

    Lentiviral

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    D95Q, D106E

    Promoter

    CamKIIa

    Available Since

    July 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus-Bik-DD-pEGFP-C1

    Plasmid

    #177429

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Threonine 33 and Serine 35 replaced with aspartic…

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. VPS35 shRNA1

    Plasmid

    #163858

    Purpose

    shRNA targeting the coding sequences of VPS35

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    VPS35 retromer complex component (VPS35 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV/EF1a-DIO-SCLM

    Plasmid

    #216759

    Purpose

    AAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoter

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    EF1a (human elongation factor 1 alpha)

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cra-GFP

    Plasmid

    #220151

    Purpose

    To track the natural Cra promoter's transcriptional dynamics

    Depositor

    Andre Sanches Ribeiro

    Article

    Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

    Insert

    Cra promoter only
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CRP-GFP

    Plasmid

    #220152

    Purpose

    To track the natural CRP promoter's transcriptional dynamics

    Depositor

    Andre Sanches Ribeiro

    Article

    Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

    Insert

    CRP promoter only
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Fis-GFP

    Plasmid

    #220153

    Purpose

    To track the natural Fis promoter's transcriptional dynamics

    Depositor

    Andre Sanches Ribeiro

    Article

    Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

    Insert

    Fis promoter only
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lrp-GFP

    Plasmid

    #220158

    Purpose

    To track the natural Lrp promoter's transcriptional dynamics

    Depositor

    Andre Sanches Ribeiro

    Article

    Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

    Insert

    Lrp promoter only
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MarA-GFP

    Plasmid

    #220159

    Purpose

    To track the natural MarA promoter's transcriptional dynamics

    Depositor

    Andre Sanches Ribeiro

    Article

    Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

    Insert

    MarA promoter only
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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