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UMI-sgRNA-TF

Pooled Library

#234819

Purpose

Unique molecular identifiers-linked sgRNA library targeting transcription factors (TFs) in mice.

Depositor

Benjamin Ebert

Article

Loke et al Blood. 2025 Jun 10:blood.2024028033. doi

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
BARBEKO sgRNA library

Pooled Library

#174163

Purpose

Cytosine base editors for genome-scale knockout screens in human cells. Targets gene start codons, splice sites, or introduces premature termination codons.

Depositor

Wensheng Wei

Article

Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only
UMI-sgRNA-CH

Pooled Library

#234820

Purpose

Unique molecular identifiers-linked sgRNA library targeting chromatin factors (CH) in mice.

Depositor

Benjamin Ebert

Article

Loke et al Blood. 2025 Jun 10:blood.2024028033. doi

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only
pLJM1-EGFP

Plasmid

#19319

Purpose

3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-lux

Plasmid

#64963

Purpose

mini-Tn7 luxCDABE transcriptional fusion vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

lux operon

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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T444T

Plasmid

#113081

Purpose

Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

Depositor

Tibor Vellai

Article

Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

Type

Empty backbone

Use

RNAi

Expression

Bacterial

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Efg1-N-PrLD

Plasmid

#216397

Purpose

Expresses 6xHis-tagged N-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-N-PrLD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-554

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC18R6KT-mini-Tn7T-Km

Plasmid

#64969

Purpose

mini-Tn7 base vector with transcriptional terminator and oriT

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFLP3

Plasmid

#64946

Purpose

Flp recombinase expression vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

Flp recombinase

Expression

Bacterial

Promoter

lambda promoter/temperature-sensitive Lambda cI r…

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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PCS2+Cas9-mSA

Plasmid

#103882

Purpose

Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

CRISPR

Tags

MSA (monomeric streptavidin

Mutation

none

Promoter

SP6

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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mApple-N1

Plasmid

#54567

Purpose

Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Type

Empty backbone

Tags

mApple

Expression

Mammalian

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pET-pro-siRNA-V2

Plasmid

#111086

Purpose

For production of recombinant siRNAs (pro-siRNAs) in E. coli.

Depositor

Linfeng HUANG

Article

Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

Type

Empty backbone

Use

RNAi

Expression

Bacterial

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMMB67EH-YFP

Plasmid

#90104

Purpose

YFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YFP

Use

Broad-host range

Expression

Bacterial

Promoter

Ptac

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_dCas9-5xGCN4_Hygro

Plasmid

#235572

Purpose

Dox-inducible expression of dCas9-GCN4 & genomic integration

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

dCas9-5xGCN4

Use

CRISPR

Mutation

D10A; H840A

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pTOX6

Plasmid

#127451

Purpose

Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

tse2 toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 GFP shRNA

Plasmid

#30323

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

GFP shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 2, 2011

Availability

Academic Institutions and Nonprofits only

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mCherry-BAF

Plasmid

#172447

Purpose

Expression of nuclear protein BAF with an N-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

BAF

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Promoter

2xCLEAR

Available Since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

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psfaMs-Tn5

Plasmid

#183637

Purpose

Expresses anti-mouse nanobody fused with Tn5

Depositor

Goncalo Castelo-Branco

Article

Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

Insert

anti mouse secondary nanobody fused to Tn5

Tags

anti-mouse nanobody

Expression

Bacterial

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pR26-CAG-H2B-miRFP703

Plasmid

#112853

Purpose

Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

H2B-miRFP703

Expression

Mammalian

Promoter

CAG

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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