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pFA6a-HTB-kanMX6

Plasmid

#26875

Depositor

Peter Kaiser

Publication

Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

Insert

HTB tag

Use

Pcr tagging yeast

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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mCD4 e/p Sal-(hCD2)

Plasmid

#24070

Depositor

Dan Littman

Publication

Sawada et al Cell. 1994 Jun 17. 77(6):917-29.

Insert

CD2 (CD2 Human)

Tags

mCD 4 promoter

Expression

Mammalian

Available since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pVC-Tol2-Pax3a_enh5-E1b:eGFP-Tol2

Plasmid

#119061

Purpose

Fluorescent vector with zebrafish pax3a enhancer 5; Tol2 version

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

pax3a_enh5-E1b:eGFP

Expression

Bacterial

Promoter

E1b minimal promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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p414ADH_Hsp82_CycTER

Plasmid

#61712

Purpose

yeast plasmid with Hsp82

Depositor

Dan Bolon

Publication

Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

Insert

6xHis-Hsp82 (HSP82 Budding Yeast)

Tags

6xHis

Expression

Yeast

Promoter

ADH1

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19_dTAG-KANSL3-targeting

Plasmid

#170755

Purpose

Donor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locus

Depositor

Kristian Helin

Publication

Damhofer et al STAR Protoc. 2021 Jun 2;2(2):100575. doi

Insert

BSD-P2A-2xHA-dTAG

Use

Mouse Targeting

Tags

BSD-P2A-2xHA-dTAG

Mutation

Phenylalanine 36 to valine in FKBP12

Available since

June 23, 2021

Availability

Academic Institutions and Nonprofits only

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pISH-Gap43

Plasmid

#74336

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

Insert

growth associated protein 43 (Gap43 Mouse)

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5 FRT TO GFP MPS1 K-R

Plasmid

#59819

Purpose

Allows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Publication

Tighe et al J Cell Biol. 2008 Jun 16;181(6):893-901.

Insert

Mps1 (TTK Human)

Tags

EmGFP

Expression

Mammalian

Mutation

KD, catalytically inactive, D664A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSK23

Plasmid

#72851

Purpose

template for C-terminal eGFP tagging, Puromycin-resistance

Depositor

Klaus Foerstemann

Publication

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Expression

Insect

Promoter

copia

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLpA-DN.JNK2

Plasmid

#51943

Purpose

Adenoviral vector for dominant negative JNK2 expression

Depositor

Jeffery Molkentin

Publication

Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.

Insert

DN JNK2 (Mapk9 Mouse)

Use

Adenoviral

Tags

HA

Mutation

dominant negative: the dual phosphorylation moti…

Promoter

CMV

Available since

May 29, 2014

Availability

Academic Institutions and Nonprofits only
pLL3.7-EF-EYFP-YAP1_S94A-PolyA

Plasmid

#112286

Purpose

Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

Depositor

Erik Sahai

Publication

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Mutation

Human YAP1-2gamma with Serine 94 to Alanine

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCjedCas9

Plasmid

#172216

Purpose

Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells

Depositor

John van der Oost

Publication

Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

Insert

catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system

Tags

Histag

Mutation

changed Asp 8 to Ala and His 559 to Ala

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (Delta Pest1)

Plasmid

#78096

Purpose

mammalian expression of GADD34 Delta Pest1

Depositor

Shirish Shenolikar

Publication

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Tags

FLAG

Expression

Mammalian

Mutation

deletion of PEST1

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pFGM1

Plasmid

#64949

Purpose

source of GmR FRT cassette

Depositor

Herbert Schweizer

Publication

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-GmR-FRT

Expression

Bacterial

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR2.1 mNFATc2

Plasmid

#17858

Depositor

Jerry Crabtree

Publication

Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

Insert

NFATc2 (Nfatc2 Mouse)

Expression

Bacterial

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-Nedd-4/2522

Plasmid

#11466

Depositor

Allan Weissman

Publication

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4-1 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 52-777

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLR5-CBh-dCas9-hEzh2-IRES-Hyg

Plasmid

#122375

Purpose

Expresses dCas9-hEzh2-IRES-Hyg in mammalian cells

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

CBh-dCas9-hEzh2-IRES-Hyg-pA

Use

Piggybac

Expression

Mammalian

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 Nodamura B2

Plasmid

#19716

Depositor

Christopher Sullivan

Publication

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Tags

EGFP

Expression

Mammalian

Mutation

2 silent point mutations

Available since

June 23, 2011

Availability

Academic Institutions and Nonprofits only
H2B-mNeonGreen-IRESpuro2

Plasmid

#183745

Purpose

Expresses H2B-mNeonGreen

Depositor

Daniel Gerlich

Publication

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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Stargazin-mCherry-FRB

Plasmid

#172443

Purpose

Expression of plasma membrane protein Stargazin tagged with mCherry-FRB

Depositor

Stephen Royle

Publication

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

Cacng2 (Cacng2 Mouse)

Tags

mCherry-HA-FRB

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-YY1

Plasmid

#169018

Purpose

Expression of YY1 driven by CMV promoter

Depositor

Claes Wadelius

Publication

Pan et al Life Sci Alliance. 2021 Jun 7;4(7). pii:

Insert

human YY1 (YY1 Human)

Expression

Mammalian

Promoter

CMV

Available since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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