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We narrowed to 58,897 results for: plasmid

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Showing: 2421 - 2440 of 58897 results

  2. lentiCRISPR - EGFP sgRNA 2

    Plasmid
    #51761
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    51761-plasmid-map-sequence-id-79377

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  3. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    91854-plasmid-map-sequence-id-176529

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  4. pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

    Plasmid
    #101230
    Purpose
    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
    Depositor
    Jennifer Doudna, Keith Joung
    Publication
    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature
    Insert
    SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A
    Tags
    10x His, MBP, and TEV site
    Expression
    Bacterial
    Mutation
    C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…
    Promoter
    T7
    Available since
    Nov. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    101230-plasmid-map-sequence-id-198024

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  6. pAAV-CAG-SomaGCaMP6f2 (AAV8)

    Viral prep
    #158757-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CAG-SomaGCaMP6f2 (#158757). In addition to the viral particles, you will also receive purified pAAV-CAG-SomaGCaMP6f2 plasmid DNA. CAG-driven expression of soma-targeting GCaMP6f2. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Publication
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Promoter
    CAG promoter
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only

  8. pAAV.hSyn-FLEX.iGABASnFR.F102G (AAV1)

    Viral prep
    #112164-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.hSyn-FLEX.iGABASnFR.F102G (#112164). In addition to the viral particles, you will also receive purified pAAV.hSyn-FLEX.iGABASnFR.F102G plasmid DNA. Synapsin-driven, Cre-dependent iGABASnFR.F102G GABA sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Publication
    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi
    Promoter
    Syn
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  9. eSpCas9(1.1)_No_FLAG_ATP1A1_G3_Dual_sgRNA

    Plasmid
    #86613
    Purpose
    Vector for tandem expression of ATP1A1 G3 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid
    Depositor
    Yannick Doyon
    Publication
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 G3 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    Use
    CRISPR; Co-selection via hdr using ouabain
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    9 Articles
    86613-plasmid-map-sequence-id-168851

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  13. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV9)

    Viral prep
    #55645-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available since
    June 8, 2020
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV-Ef1a-fDIO mCherry (AAV1)

    Viral prep
    #114471-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Flp-dependent)
    Available since
    Dec. 3, 2019
    Availability
    Academic Institutions and Nonprofits only

  15. pAAV-hSyn-GRAB_DA1m (AAV9)

    Viral prep
    #113049-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-GRAB_DA1m (#113049). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_DA1m plasmid DNA. Synapsin-driven expression of GRAB-DA1m dopamine sensor in neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Yulong Li
    Publication
    Sun et al Cell. 2018 Jul 12;174(2):481-496.e19. do
    Promoter
    Syn
    Available since
    Nov. 19, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. pAC-ZEAXipi

    Plasmid
    #53287
    Purpose
    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli
    Depositor
    Francis X Cunningham Jr
    Publication
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtE, idi, crtY, crtI, crtB, crtZ
    Use
    Low copy number bacterial cloning vector
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    53287-plasmid-map-sequence-id-82991

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Showing: 2421 - 2440 of 58897 results