We narrowed to 6,366 results for: wus

1. pCXLE-mCherry

   Plasmid

   #193293

   Purpose

   episomal vector expressing mCherry fluorescent protein

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. delCMV-mCherry-2x-Rhotekin-GBD

   Plasmid

   #214142

   Purpose

   Rho activity sensor

   Depositor

   Leif Dehmelt

   Article

   Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

   Insert

   3xRhotekin-GBD (Rtkn Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Only the GTPase binding domain (GBD) is included

   Promoter

   delCMV

   Available Since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-kanMX6-P3nmt1-GFP

   Plasmid

   #39289

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus

   Promoter

   Available Since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA11-pMa-sgTS3-17xMS2V5-sgTS3

   Plasmid

   #199442

   Purpose

   CRISPR donor plasmid to insert 17XMS2V5 into the ribosomal DNA region in dependent of homology arms

   Depositor

   Baohui Chen

   Article

   Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

   Insert

   HITI donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. sgLenti-orthogonal

   Plasmid

   #105997

   Purpose

   Dual Streptococcus pyogenes and Staphylococcus aureus sgRNA expression vector

   Depositor

   Mike McManus

   Article

   Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral; S.pyogenes sgrna expressio…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-EGFP

   Plasmid

   #91975

   Purpose

   EGFP reporter cell line control (no miR-19 sites)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a_NEUROD1_P2A_Hygro_Barcode

   Plasmid

   #120466

   Purpose

   Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   NEUROD1 (NEUROD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-3xFlag

   Plasmid

   #132403

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5'-BirA-FLAG GFP minimal CMV pCDNA5

   Plasmid

   #212087

   Purpose

   LR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Insert

   GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-ihSyn1-tTA

    Plasmid

    #99120

    Purpose

    An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    ihSyn1

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-Gsdmd-NT 4A

    Plasmid

    #80952

    Purpose

    Expresses Gsdmd-NT 4A mutant in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-NT 4A (Gsdmd Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

    Plasmid

    #132411

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRfxCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R239A; H244A; R858A; H863A

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SpyCatcher

    Plasmid

    #223633

    Purpose

    Protein purification of SpyCatcher003 (S49C) with 6xHis tag from E. coli

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    SpyCatcher003 S49C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR

    Plasmid

    #176311

    Purpose

    Expresses NLS-pMag-CreC60 with tagBFP marker

    Depositor

    Yingxiao Wang

    Article

    Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

    Insert

    tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1alpha

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-fDIO-mNeonGreen

    Plasmid

    #99133

    Purpose

    An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Has Service

    AAV1

    Insert

    fDIO-mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHAGE-IRES-puro-NLS-dPspCas13b-2xmNeongreen-NLS-3xFlag

    Plasmid

    #132402

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-BoxB

    Plasmid

    #92004

    Purpose

    Expresses firefly luciferase with BoxB sequences in 3' UTR

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    Firefly Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mCherry-C1-TGAT

    Plasmid

    #84334

    Purpose

    Expresses TGAT tagged with mCherry

    Depositor

    Dorus Gadella

    Article

    van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

    Insert

    TGAT (TRIO Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Dec. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGGS-FLAG-CaMKIID

    Plasmid

    #186827

    Purpose

    Expresses FLAG-CaMKIID in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    Camk2d (Camk2d Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV_EF1a_FLAG-Rheb(Q64L)-P2A-Hygro_Barcode

    Plasmid

    #170229

    Purpose

    Barcoded lentiviral vector to express Rheb (Q64L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Q64L

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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