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pCHKU27.1-2.2

Plasmid

#111048

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pUC19-E7H313

Plasmid

#103104

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

E7H313(Cas9 coding gene from Sutterella wadsworthensis 3_1_45B)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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PA-mCherry-miniSOG-H2B-C-10

Plasmid

#57789

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pEMS1207

Plasmid

#29104

Purpose

Expression of the eGFP reporter gene was not detected in the brain and eye.

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

Insert

Ple235

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-NLS

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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miniSOG-LaminB1-10

Plasmid

#57771

Purpose

Localization: Nuclear Envelope, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

LaminB1 (LMNB1 Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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miniSOG-Mito-7

Plasmid

#57773

Purpose

Localization: Mitochondria, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Mito (COX8A Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pFRT-DHX36iso1E335A

Plasmid

#159587

Purpose

Express N-ter FLAG HA tagged DHX36 isoform 1 with E335A mutation in mammalian cells

Depositor

Markus Hafner

Article

Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

Insert

DHX36 (DHX36 Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

E335A

Promoter

CMV

Available Since

May 29, 2021

Availability

Academic Institutions and Nonprofits only

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TRPML1-G-GECO1.2-ERES

Plasmid

#207144

Purpose

Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Mcoln1 (MCOLN1 Human)

Tags

ER-Export Signal (ERES) and G-GECO1.2

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pHtrA-TEV-His12

Plasmid

#137036

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi HtrA with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66500.2 (BB_0104 Borrelia burgdorferi B31)

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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miniSOG-Actin-7

Plasmid

#57761

Purpose

Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Actin (ACTB Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pEGFP-sfCherry11x4-ß-actin

Plasmid

#70220

Purpose

Expresses sfCherry11x4-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11x4-actin (ACTB Human)

Tags

sfCherry11x4

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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HA-Rab11-DN (S25N)

Plasmid

#101046

Purpose

Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

RAB11 (RAB11A Human)

Tags

HA

Expression

Mammalian

Mutation

S25N

Promoter

CMV

Available Since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-sfCherry11-ß-actin

Plasmid

#70221

Purpose

Expresses sfCherry11-ß-actin in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

sfCherry11-actin (ACTB Human)

Tags

sfCherry11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His beta4

Plasmid

#16039

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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Is3xP3-mCherry

Plasmid

#220091

Purpose

Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Promoter consists of 3 Pax 6 sites from 3xP3 with a minimal IsHSP70 promoter. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)

Depositor

Monika Gulia-Nuss

Article

Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

Insert

3 Pax6 binding sites from 3xP3 with minimal HSP70 promoter from Ixodes scapularis

Promoter

Is3xP3

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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HA-Rab8a-DN (T22N)

Plasmid

#101049

Purpose

Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

Depositor

Ken-Ichi Takemaru

Article

Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

Insert

Rab8a (RAB8A Human)

Tags

HA

Expression

Mammalian

Mutation

T22N

Promoter

CMV

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

Plasmid

#172104

Purpose

Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEpicVector3mC_Puro-T2A-mCherry-VSVg-ProtC-FLAG

Plasmid

#162104

Purpose

mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

Depositor

Julien Sage

Article

Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

Insert

Puromycin resistant; mCherry linked to VSVg-ProtC-FLAG

Use

Lentiviral

Tags

VSVg-ProtC-FLAG

Mutation

WT

Promoter

EF1A

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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miniSOG-Golgi-7

Plasmid

#57768

Purpose

Localization: Golgi, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Golgi (B4GALT1 Human)

Tags

miniSOG

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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SOX17-NLS-tdTomato-EPG

Plasmid

#210466

Purpose

Donor plasmid for knock-in NLS-tdTomato into the human SOX17 locus

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

SOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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