We narrowed to 165,918 results for: addgene

1. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

   Plasmid

   #75295

   Purpose

   An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

   Depositor

   Brandon Harvey

   Insert

   eGFP-2A-mKv1.2 (Kcna2 Synthetic, Mouse)
   

   Use

   AAV

   Promoter

   human SYN1

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. lag-2 promoter:GFP (pJK590)

   Plasmid

   #26814

   Depositor

   Judith Kimble

   Article

   Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

   Insert

   lag-2 promoter
   

   Tags

   GFP

   Expression

   Worm

   Available Since

   Dec. 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv2-Stuffer-HepmTurquoise2

   Plasmid

   #192826

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mTurquoise2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. MXS_PGK::CreERT2-bGHpA

   Plasmid

   #62444

   Purpose

   MXS_chaining vector with PGK::CreERT2-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX459_gRNA pAS single_hspCas9

   Plasmid

   #193311

   Purpose

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

   Insert

   Cas9 from S. pyogenes and U6-pAS single sgRNA (V2.0)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   not applicable

   Available Since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. FUap97GW (C4)

   Plasmid

   #74020

   Purpose

   lentiviral expression of SAP97alpha-EGFP fusion

   Depositor

   Weifeng Xu, Robert Malenka, Oliver Schluter

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   SAP97 alpha
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   hUbC

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-BC-CMV-Puro

   Plasmid

   #127169

   Purpose

   PCR template for CMV-puro resistance cassette

   Depositor

   Paul Blainey

   Article

   Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. MXS_CMV::CreERT2-bGHpA

   Plasmid

   #62443

   Purpose

   MXS_chaining vector with CMV::CreERT2-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPR_eGFP_369

   Plasmid

   #111823

   Purpose

   Knockout eGFP

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting eGFP 349-369
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCM-HA-Gas1

    Plasmid

    #38313

    Depositor

    Susan Lindquist

    Article

    McLellan et al ACS Chem Biol. 2012 Jun 22.

    Insert

    Gas1 (GAS1 Budding Yeast)
    

    Tags

    2xHA

    Mutation

    contains promoter region

    Promoter

    Endogenous

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. EmptyTranscriptionalReporter

    Plasmid

    #62453

    Purpose

    MXS_chaining vector with 2 cassettes

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Inserts

    CMVmin::NLS-Citrine-bGHpA
    CMV::NLS-Cherry-bGHpA
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pN-PURO-PTP

    Plasmid

    #51711

    Purpose

    contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Available Since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. M4758 KanMX::URA3 Disruptor Converter

    Plasmid

    #51688

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to URA3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    URA3 (URA3 Budding Yeast)
    

    Use

    Yeast marker swap

    Expression

    Yeast

    Available Since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR_eGFP_314

    Plasmid

    #111822

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 294-314
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. B1979

    Plasmid

    #59181

    Purpose

    Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    Blasticidin S resistance
    

    Expression

    Worm

    Promoter

    rpl-28

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNM3689

    Plasmid

    #154111

    Purpose

    miniMos single component RMCE landing site vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    loxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
    

    Use

    Minimos

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MXS_CMV::NeoR-bGHpA

    Plasmid

    #62437

    Purpose

    MXS_chaining vector with CMV::NeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Neomycin with CMV Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDOC-K-glmS-lacIZ

    Plasmid

    #158061

    Purpose

    Donor plasmid for insertion of the lacIZ operon and lac promoter into the S. Typhimurium chromosome

    Depositor

    Mark Webber

    Article

    Holden et al Microbiology (Reading). 2020 Nov 23. doi

    Insert

    lacIZ operon
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CXXC1

    Plasmid

    #32045

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CXXC1 (CXXC1 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    CXXC domain (amino acids residues 161-222)

    Available Since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. MXS_PGK::PuroR-bGHpA

    Plasmid

    #62440

    Purpose

    MXS_chaining vector with PGK::PuroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Puromycin with PGK Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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