We narrowed to 13,608 results for: crispr cas9

1. Fuw-dCas9-Dnmt3a_IM

   Plasmid

   #84478

   Purpose

   Lentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a_IM
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a/Cas9-Cys

   Plasmid

   #53261

   Purpose

   Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine

   Depositor

   Hyongbum Kim

   Article

   Ramakrishna et al Genome Res. 2014 Apr 2.

   Insert

   Cas9-Cys
   

   Tags

   6X His tag, HA epitope, and NLS

   Expression

   Bacterial

   Mutation

   Additional Cysteine is added to the C-terminal re…

   Promoter

   T7 Promoter

   Available Since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-10xSunTag(22aa)

   Plasmid

   #215745

   Purpose

   Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

   Depositor

   Albert Jeltsch

   Article

   Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

   Insert

   dCas9-10xSunTag
   

   Expression

   Mammalian

   Promoter

   chicken beta-actin promoter

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX303-ABI1-dCas9

   Plasmid

   #154474

   Purpose

   Expresses ABI1 fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ABI1 (ABI1 Mustard Weed)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Includes ABI1 aa 126-423, D143A

   Promoter

   CMV

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-P3-evoCjCas9-ABE8e_gRNA

   Plasmid

   #202559

   Purpose

   P3-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

   Depositor

   Gerald Schwank

   Article

   Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

   Insert

   evoCjCas9-ABE8e
   

   Use

   AAV

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS315e_pGal-dCas9-PmCDA1

   Plasmid

   #79615

   Purpose

   Expresses dCas9-PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Tags

   PmCDA1

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX459-HypaCas9-H2BC11_sgRNA

   Plasmid

   #183883

   Purpose

   pX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   H2BC11 sgRNA spacer (H2BC11 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-HypaCas9-MYH10_sgRNA

   Plasmid

   #183887

   Purpose

   pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   MYH10 sgRNA spacer (MYH10 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti spCas9 T2A iRFP670 P2A puro

   Plasmid

   #122182

   Purpose

   The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

   Depositor

   Raphael Gaudin

   Inserts

   spCas9 (NEWENTRY )
   iRFP670
   

   Use

   Lentiviral

   Tags

   T2A

   Expression

   Mammalian

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti spCas9 T2A mNeonGreen P2A puro

    Plasmid

    #122183

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a mNeonGreen fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9 (NEWENTRY )
    mNeonGreen
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti spCas9 T2A TagRFP-T P2A puro

    Plasmid

    #122200

    Purpose

    The plasmid codes for a Flag-spCas9 protein, a TagRFP-T fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

    Depositor

    Raphael Gaudin

    Inserts

    spCas9
    TagRFP-T
    

    Use

    Lentiviral

    Tags

    T2A

    Expression

    Mammalian

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

    Plasmid

    #228251

    Purpose

    pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
    

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-dCas9-KRAB-blast

    Plasmid

    #89567

    Purpose

    Plasmid expression dCas9 protein in fusion with KRAB domain

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    Blasticidin resistance gene and KRAB

    Mutation

    D10A and H840A

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-Flag-wtSpCas9-H840A

    Plasmid

    #80453

    Purpose

    SpCas9 mammalian expression vector

    Depositor

    Ervin Welker

    Article

    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

    Insert

    SpCas9_H840A
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT3TS-HiFi-nCas9n-A72

    Plasmid

    #125646

    Purpose

    micro-injection of poly-adenylated HiFi-nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    HiFi-nCas9n
    

    Use

    Rna in vitro transcription vector

    Mutation

    R691A from wild-type Cas9

    Promoter

    T3

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc-sgRNA-Cas9.scr_2

    Plasmid

    #190704

    Purpose

    Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Scrambled sgRNA 2
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    Drosophila U6

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-SaCas9-U6-sgKcnab2

    Plasmid

    #192795

    Purpose

    Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

    Depositor

    Marta Soden

    Article

    Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

    Insert

    sgKcnab2
    

    Use

    AAV and CRISPR

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3

    Plasmid

    #231568

    Purpose

    Expresses components of the LOOMINA optogenetic transcriptional control system.

    Depositor

    Dominik Niopek

    Article

    Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

    Insert

    All-in-one LOOMINA construct
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEX-SaCas9-U6-sgKcna2

    Plasmid

    #192796

    Purpose

    Encodes SaCas9 and an sgRNA targeting mouse Kcna2

    Depositor

    Marta Soden

    Article

    Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

    Insert

    sgKcna2
    

    Use

    AAV and CRISPR

    Promoter

    U6

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEX-SaCas9-U6-sgKcna6

    Plasmid

    #192797

    Purpose

    Encodes SaCas9 and an sgRNA targeting mouse Kcna6

    Depositor

    Marta Soden

    Article

    Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

    Insert

    sgKcna6
    

    Use

    AAV and CRISPR

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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