We narrowed to 7,064 results for: mme

1. pMB66

   Plasmid

   #47946

   Purpose

   Expresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoter

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

   Expression

   Worm

   Mutation

   Promoter

   hsp-16.48

   Available since

   Dec. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. TOPO mCherry

   Plasmid

   #68490

   Purpose

   TOPO construct expressing mCherry for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   mCherry
   

   Use

   Gmap

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJB01

   Plasmid

   #111957

   Purpose

   Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

   Depositor

   Matthew Good

   Article

   Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

   Insert

   RFP
   

   Use

   Tags

   HaloTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PAX6 TALEN R

   Plasmid

   #105525

   Purpose

   Knock in H2B-GFP into the human PAX6 locus

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

   Insert

   Pax6 TALEN R
   

   Use

   TALEN

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PAX6 TALEN L

   Plasmid

   #109034

   Purpose

   Knock in H2B-GFP into the human PAX6 locus

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

   Insert

   Pax6 TALEN L
   

   Use

   TALEN

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA-lynD3cpV

   Plasmid

   #37472

   Depositor

   Amy Palmer, Roger Tsien

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D3cpv second generation cameleon (calcium sensor)
   

   Use

   Tags

   the myristoylation and palmitoyla- tion sequence …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pLBH546_Tet-Cas14b1Locus

   Plasmid

   #112503

   Purpose

   Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL-GFPGO-PGK-Neo

   Plasmid

   #136890

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLBH547_Tet-Cas14b2Locus

   Plasmid

   #112504

   Purpose

   Expresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b2
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Tet

   Available since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Spy0469

    Plasmid

    #159996

    Purpose

    Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

    Depositor

    Mark Howarth

    Article

    Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

    Insert

    Spy0469 (24-389)
    

    Use

    Tags

    His6-tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. TOPO PuroR

    Plasmid

    #68447

    Purpose

    TOPO construct expressing PuroR for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRYG

    Plasmid

    #113642

    Purpose

    Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    mRFP1-linker-sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMB62

    Plasmid

    #47944

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. TOPO ZeoR

    Plasmid

    #68446

    Purpose

    TOPO construct expressing ZeoR for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Zeocin Resistance
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. TOPO HygroR

    Plasmid

    #68445

    Purpose

    TOPO construct expressing HygroR for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Hygromycin Resistance
    

    Use

    Gmap

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Plac

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR1054

    Plasmid

    #71822

    Purpose

    Express Streptococcus pyogenes nCas9 (D10A) nickase carrying two C-terminal SV40 NLS

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

    Insert

    T7pr_6xHis-MBP-TEV-SPynCas9 (D10A)-2xNLS
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. FFiBlast p5

    Plasmid

    #68470

    Purpose

    Inverted and floxed blasticidin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Blasticidin Resistance
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Inverted

    Promoter

    LTR

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR SD Age HsSAS-6

    Plasmid

    #46381

    Purpose

    For expression of C-terminal fusions.

    Depositor

    Pierre Gonczy

    Article

    Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

    Insert

    SAS-6 (SASS6 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. 3534 pcDNA3 Bax

    Plasmid

    #8783

    Depositor

    Stanley Korsmeyer

    Article

    Oltvai et al Cell 1993 Aug 27;74(4):609-19.

    Insert

    Bax (Bax Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pCY 3010-02

    Plasmid

    #36210

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    yeast-enhanced (yE) polylinker
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-Cerulean-hphMX4

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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