We narrowed to 54,125 results for: tra

1. pENTR1A_no_ccDB_mUty

   Plasmid

   #158570

   Purpose

   V5-tagged sequence of mUty cloned into entry vector for recombination

   Depositor

   Christian Deschepper

   Article

   Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

   Insert

   Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS31: pTwinStrep-SUMO-PmcTnsAB

   Plasmid

   #168164

   Purpose

   PmcTnsAB expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsAB
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (29-339)

   Plasmid

   #136544

   Purpose

   Expresses ANXA2 chimeras in bacterial cells

   Depositor

   Laszlo Nyitray

   Article

   Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

   Type

   Empty backbone

   Use

   Tags

   ANXA2 (29-339) and His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-beta1,4 GT-1

   Plasmid

   #11872

   Depositor

   Michiko Fukuda

   Article

   Masri et al Biochem Biophys Res Commun. 1988 Dec 15.

   Insert

   beta 1,4 galactosyltransferase (B4GALT1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pUCSh3-mRFP

   Plasmid

   #170983

   Purpose

   Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   mRFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

   Plasmid

   #136546

   Purpose

   Expresses ANXA2 chimeras in bacterial cells

   Depositor

   Laszlo Nyitray

   Article

   Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

   Type

   Empty backbone

   Use

   Tags

   ANXA2 (23-339) and His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3)

   Plasmid

   #182370

   Purpose

   Dendra2-based translation reporter

   Depositor

   Chunghun Lim

   Article

   Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

   Insert

   mtIF3 (Mtif3 Mouse)
   

   Use

   Tags

   Pal-Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ptagBFP-LacI-NLS-VP16

   Plasmid

   #103837

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-LacI-NLS-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTC-CMV-Tet

   Plasmid

   #85577

   Purpose

   Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (CMV promoter) in mouse liver

   Depositor

   Ursula Ehmer

   Article

   Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

   Insert

   CreER
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   ApoE.HCR.hAAT

   Available since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS10: pTns(AvCAST)_ΔTnsD

    Plasmid

    #168143

    Purpose

    Inducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-SERTG56A -myc

    Plasmid

    #107459

    Purpose

    Mammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G56A conferring wildtype phenotype

    Depositor

    Erik Procko

    Article

    Young et al bioRxiv 2021.04.19.440442

    Insert

    Serotonin Transporter (SERT) (SLC6A4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G56A

    Promoter

    CMV

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZF-BCB-GCN

    Plasmid

    #127476

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZF-BCB-PDZ

    Plasmid

    #127478

    Purpose

    coding sequence for synthetic zinc finger ZF-BCB-PDZ

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    

    Use

    Cell-free transcription–translation

    Tags

    alpha 1-syntrophin PDZ domain

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. YFP-DAT R60A

    Plasmid

    #90247

    Purpose

    Expresses YFP-DAT R60A mutant in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Ma et al Sci Rep. 2017 Jul 14;7(1):5399. doi: 10.

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutated R60 to A in human DAT cDNA

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZF-ADD-L

    Plasmid

    #127477

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD-L

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
    

    Use

    Cell-free transcription–translation

    Tags

    PDZ cognate peptide ligand (VKESLV)

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZF-ADD-GCN

    Plasmid

    #127475

    Purpose

    coding sequence for synthetic zinc finger ZF-ADD-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Expression

    Mutation

    Promoter

    T7

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-X3

    Plasmid

    #115568

    Purpose

    Synthetic acetylation substrate consisting of GFP fusion with 3 repeats of the Gcn5 consensus motif

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Three repeats of the Gcn5 consensus motif are tag…

    Promoter

    ADH1

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pR-Avr

    Plasmid

    #22650

    Depositor

    Lynn Hudson

    Article

    Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

    Insert

    Myelin Transcription Factor 1 (Myt1 Rat)
    

    Use

    Cloning and rna synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pmMyt1

    Plasmid

    #22713

    Depositor

    Lynn Hudson

    Article

    Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

    Insert

    Myelin Transcription Factor 1 (Myt1 Mouse)
    

    Use

    Cloning vector tt/aa

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCT162: pTwinStrep-bdSUMO-AjTn6022_TnsF

    Plasmid

    #200865

    Purpose

    AjTn6022_TniQ expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

    Insert

    AjTn6022_TnsF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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