We narrowed to 6,366 results for: wus;

1. EF1a_SOX10_P2A_Hygro_Barcode

   Plasmid

   #120479

   Purpose

   Barcoded lentiviral vector to express SOX10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SOX10 (SOX10 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerald-Tubulin-C-18

   Plasmid

   #54292

   Purpose

   Localization: Microtubules, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Gao et al Cell. 2012 Dec 7;151(6):1370-85. doi: 10

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. NDV-HN-SpyTag

   Plasmid

   #160000

   Purpose

   Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   NDV-HN-SpyTag
   

   Use

   Tags

   His6-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta-actin promoter

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-hSyn-Galpha-i1 ONE-GO

   Plasmid

   #204333

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-i1 ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pDestTol2-actinb-kcna1a-IRES-EGFP

   Plasmid

   #164965

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcna1a and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcna1a (kcna1a Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   actinb (actb2) promoter

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_MITF_P2A_Hygro_Barcode

   Plasmid

   #120460

   Purpose

   Barcoded lentiviral vector to express MITF in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MITF (MITF Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CAG-Cremyc-2A-GFP

   Plasmid

   #116879

   Purpose

   Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

   Depositor

   Qiang Lu

   Article

   Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

   Insert

   Cremyc-2A-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR3-FLAG-TRAF6-C70A

   Plasmid

   #43926

   Depositor

   Bryant Darnay

   Article

   Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

   Insert

   TNF Receptor-Associated Factor 6 (Traf6 Mouse)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   C70A

   Promoter

   Available Since

   Jan. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac_human-IL-18

   Plasmid

   #214338

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-DIO-mNeonGreen

    Plasmid

    #99132

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mNeonGreen

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    DIO-mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET11a-AldoA

    Plasmid

    #224925

    Purpose

    Inducible expression of human aldolase A

    Depositor

    Liskin Swint-Kruse

    Article

    Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

    Insert

    Aldolase A (ALDOA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag

    Plasmid

    #132401

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-G-GECO1.1

    Plasmid

    #32445

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.1)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3 K69E/N77Y/D86G/K119I/L173Q/K380N/S404G/E43…

    Promoter

    CMV

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-IRES-puro-NLS-dLbaCas13a-EGFP-NLS-3xFlag

    Plasmid

    #132408

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dLbaCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    R600A; H605A; R1243A; H1248A

    Promoter

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ-dpcoCas9-SunTag

    Plasmid

    #158410

    Purpose

    CRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-VP64-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX305-N-dTAG-CARD8

    Plasmid

    #169989

    Purpose

    Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    dTAG

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SMVP-Cas9N-U6-gRNA M3

    Plasmid

    #80936

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLVX-hSyn-Galpha-oA ONE-GO

    Plasmid

    #204335

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-oA ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pBABR IRFP-Bcd-LEXY

    Plasmid

    #182595

    Purpose

    Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

    Depositor

    Jared Toettcher

    Article

    Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

    Insert

    Bcd (bcd Fly)
    

    Use

    Tags

    IRFP and LEXY

    Expression

    Insect

    Mutation

    Promoter

    maternal tubulin

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MTK4_002

    Plasmid

    #123811

    Purpose

    Encodes SV40 polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    SV40 pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge