We narrowed to 6,366 results for: wus;

1. EF1a_MYOD1_P2A_Hygro_Barcode

   Plasmid

   #120464

   Purpose

   Barcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   MYOD1 (MYOD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-FLAG-CaMKIIG

   Plasmid

   #186828

   Purpose

   Expresses FLAG-CaMKIIG in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2g (Camk2g Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK2_007

   Plasmid

   #123702

   Purpose

   Encodes human pEF1alpha as a Type 2 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   pEF1a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-GEX-GECO1

   Plasmid

   #32443

   Purpose

   Mammalian expression of green excitation ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEX-GECO1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3 K69E/K119I/L173Q/T223S/F315L/F368L/L372Q/K…

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-TRE-DIO-mTurquoise2

   Plasmid

   #99115

   Purpose

   An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mTurquoise2

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   DIO-mTurquoise2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.27_GRHL-SPE

   Plasmid

   #177774

   Purpose

   Luciferase reporter for GRHL

   Depositor

   Michael Ristow

   Article

   Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

   Insert

   GRHL
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-kanMX6-P41nmt1

   Plasmid

   #39281

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available Since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pFA6a-kanMX6-P81nmt1

   Plasmid

   #39282

   Depositor

   Jurg Bahler, John Pringle

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 7bp deletion (AT…

   Promoter

   Available Since

   Sept. 25, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-hSyn-Galpha-q ONE-GO

   Plasmid

   #204336

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   hSyn Galpha-q ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBAD-T-GECO1

    Plasmid

    #191616

    Purpose

    Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

    Depositor

    Robert Campbell

    Article

    Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

    Insert

    T-GECO1
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 CARD8-TEV-GFP-FLAG

    Plasmid

    #169991

    Purpose

    Mammalian purification construct for CARD8

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-SMVP-Cas9N-U6-gRNA P1

    Plasmid

    #80943

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry

    Plasmid

    #192634

    Purpose

    Lentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Minimal promoter

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK4_003

    Plasmid

    #123812

    Purpose

    Encodes rabbit beta-globin polyadenylation signal as a Type 4 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    rglb pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_Pro-IL-37

    Plasmid

    #214346

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL37 (IL37 Human)
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dnase1L3 R206C pIRES-DsRed

    Plasmid

    #98847

    Purpose

    Expresses Human Dnase1L3 with activity-reducing HUVS mutation R206C in mammalian cells along with DsRed

    Depositor

    Peter Keyel

    Article

    Shi et al Front Immunol. 2017 May 8;8:522. doi: 10

    Insert

    Dnase1L3 (DNASE1L3 Human)
    

    Use

    Tags

    IRES DsRed

    Expression

    Mammalian

    Mutation

    R206C which greatly reduces nuclease activity

    Promoter

    CMV

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-BMAL1

    Plasmid

    #186824

    Purpose

    Expresses BMAL1 in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiCRISPRv2 EGFP sgRNA2

    Plasmid

    #164933

    Purpose

    Expresses EGFP sgRNA2 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA2 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCBE4-gam

    Plasmid

    #124449

    Purpose

    Expresses dead Cas9 (dCas9)-CBE4-gam

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dCBE4-gam
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. HBT_pcoCASphi_version1

    Plasmid

    #197931

    Purpose

    Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Insert

    pcoCasphi-2
    

    Use

    CRISPR

    Tags

    FLAG tag and SV40 NLS and nucleoplasmin NLS

    Expression

    Plant

    Mutation

    Promoter

    35SPPDK promoter

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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