We narrowed to 2,568 results for: gem

1. pMGF172

   Plasmid

   #96998

   Purpose

   ish1 mCherry insertion cassette with ura4+ gene

   Depositor

   Adam Frost, Wesley Sundquist

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + ura4(…

   Promoter

   N/A

   Available Since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLexA::SRDX (GB0732)

   Plasmid

   #68184

   Purpose

   Provides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   LexA Binding Domain fused to SRDX Transcriptional Repressor
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGal4BD::BDR (GB0734)

   Plasmid

   #68185

   Purpose

   Provides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   Gal4 Binding Domain fused to BDR Transcriptional Repressor
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBOB-EF1-FastFUCCI-Puro

   Plasmid

   #86849

   Purpose

   3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

   Depositor

   Kevin Brindle, Duncan Jodrell

   Article

   Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

   Inserts

   mKO2-hCDT1(30-120)
   mAG-hGEM(1-110)
   pac (puromycin N-acetyl-transferase)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1 and PGK

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPR-HOT-P2A-Clover-BlastR

   Plasmid

   #138568

   Purpose

   Universal Cleavable Donor Plasmid for tagging with P2A-Clover-BlastR using NHEJ

   Depositor

   Hans Clevers

   Article

   Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

   Insert

   P2A-clover-BlastR
   

   Use

   CRISPR

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-FastFUCCI (JDW 1488)

   Plasmid

   #242556

   Purpose

   Gateway middle entry clone containing FastFUCCI reporter; For visualizing cell cycle state

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)
   

   Use

   Gateway subcloning

   Available Since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUPD P35S (GB0552)

   Plasmid

   #160554

   Purpose

   35s promoter for N-terminal fusions

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Nucleic Acids Res. 2017 Feb 28;45(4):219

   Insert

   35s
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJC5-4-insulator-BREreporter-insulator

   Plasmid

   #171499

   Purpose

   injections to make Bmp reporter mouse transgenic line

   Depositor

   James Briscoe

   Article

   Ivanovitch et al PLoS Biol. 2021 May 17;19(5):e3001200. d

   Insert

   Insulator BRE-MLP:H2B-Turqoise
   

   Expression

   Mammalian

   Promoter

   BRE-MLP

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

9. pISH-Ascl1

   Plasmid

   #74338

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Ascl1 (also known as Mash1). Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

   Insert

   achaete-scute family bHLH transcription factor 1 (Ascl1 Mouse)
   

   Available Since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMGF157

    Plasmid

    #96972

    Purpose

    ish1-mCherry insertion cassette with Hygro resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + hygro…

    Promoter

    N/A

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMGF170

    Plasmid

    #96997

    Purpose

    ish1-mCherry insertion cassette with NAT resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + NAT r…

    Promoter

    N/A

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-zFUCCI (JDW 1464)

    Plasmid

    #242558

    Purpose

    Gateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
    

    Use

    Gateway subcloning

    Available Since

    Aug. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

    Plasmid

    #221447

    Purpose

    Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

    Depositor

    Daniel Durocher

    Article

    Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

    Insert

    NFATC2IP (NFATC2IP Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    SPR degron: hGEM (1-110) fragment that is fused t…

    Promoter

    CMV

    Available Since

    July 3, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMGF130

    Plasmid

    #96971

    Purpose

    S. pombe lem2 knockout cassette with hygro resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    lem2 (lem2 Fission Yeast)
    

    Expression

    Bacterial

    Mutation

    whole ORF deletion and replaced with hygro resist…

    Promoter

    N/A

    Available Since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMGF169

    Plasmid

    #96996

    Purpose

    ish1 mCherry insertion cassette with G418 resistance gene

    Depositor

    Adam Frost, Wesley Sundquist

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + G418 …

    Promoter

    N/A

    Available Since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRN120

    Plasmid

    #160696

    Purpose

    BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Inserts

    CmYLCV::Luc+::AtHSP
    Nos::WUS2::PinII
    AtUbi10::STM::35S
    

    Use

    Luciferase; Gemini viral replicon, plant developm…

    Promoter

    AtUbi10, CmYLCV, and Nos

    Available Since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SMN2

    Plasmid

    #187587

    Purpose

    Express FLAG epitope and APEX2-tagged SMN2 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SMN2 (SMN2 Human)
    

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Gfp-rt-vasa

    Plasmid

    #167322

    Purpose

    Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

    Depositor

    Goro Yoshizaki

    Article

    Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

    Insert

    rt vasa 3'UTRs (ddx4 rainbow trout)
    

    Expression

    Bacterial

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. tRNA-gRNA position [D1_n-1] (GB1208)

    Plasmid

    #75409

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [D1_n-1]
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. SMN1-1

    Plasmid

    #156022

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SMN1 (SMN1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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