We narrowed to 58,513 results for: plasmid
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Plasmid#61565PurposeStandardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistantDepositorTypeEmpty backboneUseSynthetic Biology; Mini-tn5 vectorAvailable SinceJan. 7, 2015AvailabilityAcademic Institutions and Nonprofits only
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p2attPC
Plasmid#51547PurposeDICE donor plasmid without neural transcription factors.DepositorInsertmCherry
ExpressionMammalianAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-BAIAP2 wt
Plasmid#31656DepositorAvailable SinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH3U3-zif268 (omega)
Plasmid#18046DepositorInsertzif268 binding site
ExpressionBacterialMutationThe preferred binding site for zif268 is inserted…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TBG-ER-TurboID
Plasmid#149415PurposeHepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorHas ServiceAAV8InsertER-TurboID
UseAAVTagsHA and V5PromoterTBGAvailable SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)
Viral Prep#137141-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoternEFTagsEYFP (Flp-dependent)Available SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV CD68-hM4D(Gi)-mCherry (AAV1)
Viral Prep#75033-AAV1PurposeReady-to-use AAV1 particles produced from pAAV CD68-hM4D(Gi)-mCherry (#75033). In addition to the viral particles, you will also receive purified pAAV CD68-hM4D(Gi)-mCherry plasmid DNA. CD68-driven hM4D(Gi) receptor with an mCherry reporter for CNO-induced microglial inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCD68TagsmCherryAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CD
Plasmid#101921PurposeThe dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).DepositorInsertdCas9-TET1CD
UseLentiviralExpressionMammalianAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSFV-Helper1
Plasmid#92073PurposeThis is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.DepositorInsertcDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
UseHelper plasmid, for run-off transcription and rna…Available SinceJuly 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRFHUE-eGFP
Plasmid#89469PurposeGFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformationDepositorInserteGFP
UseBinary plasmid for atmt of filamentous fungiPromoterA. nidulans gpdAAvailable SinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP2135
Plasmid#72248PurposeHuman expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAGDepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, and D1135EPromoterCMVAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSP680
Plasmid#65772PurposeHuman expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135E, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7Available SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS404-PTEF-Ago1
Plasmid#22313DepositorInsertAGO1
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS405-PTEF-Dcr1
Plasmid#22314DepositorInsertDCR1
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
hIRE1a K599A
Plasmid#20745DepositorInsertIRE1 (ERN1 Human)
UseGateway Cloning and LentiviralExpressionMammalianMutationK599A, dominant negative kinase mutantAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTS118 His14-Avi-28xGS-anti ALFA tag nanobody
Plasmid#199371PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-ALFA nanobody
Tags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
eeBxb1_IVT
Plasmid#222342PurposePlasmid for evolved and engineered Bxb1 mRNA in vitro transcription templateDepositorInsertCodon-optimized evolved and engineered Bxb1
UseTemplate for in vitro transcriptionPromoterT7 (inactivated)Available SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)
Plasmid#196702PurposePlasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.DepositorInserthuman RNaseH1 wild type
ExpressionMammalianAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-GCaMP6f (AAV1)
Viral Prep#135632-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.DepositorAvailable SinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (AAV1)
Viral Prep#83897-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hDlx-GqDREADD-dTomato-Fishell-4 (#83897). In addition to the viral particles, you will also receive purified pAAV-hDlx-GqDREADD-dTomato-Fishell-4 plasmid DNA. hDlx-driven expression of GqDREADD (hM3D) and nuclear localized dTomato (physically separate) for activation of interneurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomatoAvailable SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only