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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,350 results for: 11

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Showing: 2541 - 2560 of 8350 results

  9. pJMP3665

    Plasmid
    #222351
    Purpose
    Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.
    Depositor
    Jason Peters
    Publication
    Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013
    Type
    Empty backbone
    Expression
    Bacterial
    Available since
    July 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222351-plasmid-map-sequence-id-439073

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  10. pODN-AAVS1-mNG-CAAX

    Plasmid
    #183870
    Purpose
    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183870-plasmid-map-sequence-id-362419

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  11. Flag-M4-AR-S81A

    Plasmid
    #171241
    Purpose
    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)
    Depositor
    Steven Balk
    Publication
    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.
    Insert
    3xFlag-tagged human androgen receptor Ser81Ala mutant (AR Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-Ser81Ala (AR-S81A)
    Promoter
    CMV
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171241-plasmid-map-sequence-id-389976

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  14. pUASTattb-cAMPr Y205A, R626A

    Plasmid
    #84932
    Purpose
    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.
    Depositor
    Justin Blau
    Publication
    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/
    Insert
    cAMPr Y205A R626A
    Expression
    Insect
    Mutation
    PKA-C Y205A, PKA-R R626A
    Promoter
    5X UAS
    Available since
    March 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    84932-plasmid-map-sequence-id-180302

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  17. FLT1-KDR

    Plasmid
    #86034
    Purpose
    chimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2
    Depositor
    Christie Thomas
    Publication
    Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human VEGFR1-VEGFR2 hybrid (FLT1, KDR Human)
    Tags
    HA and t-GFP
    Expression
    Mammalian
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    86034-plasmid-map-sequence-id-173914

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  18. pcDNA-NES-ZapCmR1.1

    Plasmid
    #59014
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 2541 - 2560 of 8350 results