We narrowed to 23,980 results for: CHI

1. pLenti HsATP10B_G671R/N865K

   Plasmid

   #204478

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B G671R/N865K mutants

   Depositor

   Veerle Baekelandt

   Article

   Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   G671R / N865K

   Promoter

   CMV

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti HsATP10B_E993A

   Plasmid

   #204479

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B E993A mutant

   Depositor

   Veerle Baekelandt

   Article

   Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E993A

   Promoter

   CMV

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti HsATP10B_I1038T

   Plasmid

   #204480

   Purpose

   transfer plasmid for lentiviral vector production expressing Hs ATP10B I1038T mutant

   Depositor

   Veerle Baekelandt

   Article

   Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   I1038T

   Promoter

   CMV

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS2326

   Plasmid

   #169628

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTS2321

   Plasmid

   #169626

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTS2329

   Plasmid

   #169629

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTS2322

   Plasmid

   #169627

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' Tactb insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::Tactb-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and Tactb-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTS2320

   Plasmid

   #169625

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and spacer-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTS2312

   Plasmid

   #169622

   Purpose

   Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of SEAP and iRFP and CoTC-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV / PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTS2317

    Plasmid

    #169624

    Purpose

    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP and pGL3-modulated PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV / PPGK

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCapVQ329R

    Plasmid

    #183029

    Purpose

    the patatin-like phosphodiesterase CapV variant CapVQ329R cloned in the L-arabinose inducible vector pBAD28

    Depositor

    Ute Romling

    Article

    Li et al NPJ Biofilms Microbiomes. 2022 May 11;8(

    Insert

    patatin-like phospholipase variant CapVQ329R
    

    Use

    Tags

    No

    Expression

    Bacterial

    Mutation

    glutamine 329 changed to arginine

    Promoter

    pBAD promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmirGLO-3'UTR-IL22

    Plasmid

    #153070

    Purpose

    Human IL-22 3'UTR region cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    3' UTR region of IL-22 gene (IL22 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBB0405-TEV-His12

    Plasmid

    #137040

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0405 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66795.1 (BB_0405 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRevA-TEV-His12

    Plasmid

    #137034

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi RevA with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAF07416.1 (revA Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pP13-TEV-His12

    Plasmid

    #137035

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi P13 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66426.1 (BB_0034 Borrelia burgdorferi B31)
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid

    #136656

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK

    Plasmid

    #126651

    Purpose

    B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    Lrig1 (Lrig1 Mouse)
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    C-terminal fusion of T2A-sfGFP-iCre-ERT2

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid

    #136655

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid

    #136653

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZS1[GalS-L,RbsR-L]

    Plasmid

    #60763

    Purpose

    Contains PIq driving expression GalS-L, and PIq driving expression of RbsR-L.

    Depositor

    Matthew Bennett

    Article

    Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

    Inserts

    GalS-L
    RbsR-L
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    LacI/GalR repressor chimera. LacI DBD with GalS L…

    Promoter

    Available since

    July 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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