We narrowed to 7,871 results for: 199

1. pLenti-SwitchON-sgVRK1

   Plasmid

   #199638

   Purpose

   Tamoxifen-inducible expression of sgRNA targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKM586

   Plasmid

   #19978

   Depositor

   David Waugh

   Article

   Kapust et al Protein Expr Purif. 2000 Jul . 19(2):312

   Insert

   TEV protease (TEVgp1 tobacco etch virus)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S219D mutant

   Promoter

   Available since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKI1

   Plasmid

   #199118

   Purpose

   Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

   Depositor

   Jeff Gelles

   Article

   Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

   Insert

   H6-SNAP-RapA
   

   Use

   Tags

   6xHis and SNAP-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1/2-NORAD-(A)4x

   Plasmid

   #199208

   Purpose

   1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

   Depositor

   Esther Braselmann

   Article

   Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

   Insert

   1/2-NORAD
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   4 copies of Riboglow tag after stop codon

   Promoter

   CMV

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTS114 pHAGE2 CMVtetO2 MCS-TEV-SUMOEu-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199367

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TEV-SUMOEu-ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

   Plasmid

   #199356

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiCas9-sgVRK1 #1-Blast

   Plasmid

   #199646

   Purpose

   Expresses Cas9 and sgRNA guide targeting VRK1

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A (VRK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-AU1-mTOR-L1460P

   Plasmid

   #19995

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-L1460P (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Leucine 1460 to Proline

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

9. AiP13905 - pAAV-AiE0441h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3905)

   Plasmid

   #199774

   Purpose

   enhancer AAV for expression of SYFP2 in all striatal medium spiny neurons

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Has Service

   AAV PHP.eB

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   wtGFP plus mutations F46L F64L S65G S72A M153T V1…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-6xHis-GFP(+6)

    Plasmid

    #199160

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+6)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL2_169

    Plasmid

    #199101

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGLDV_41 [11o1G]

    Plasmid

    #199070

    Purpose

    Odd assembly vector with sfGFP dropout for Odd level assemblies

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDropIn-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199195

    Purpose

    Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDropIn-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199196

    Purpose

    Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTol2_claudink:Cas9green; erbb2_gRNA171

    Plasmid

    #199339

    Purpose

    pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    claudink
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. zCREST3:Cas9green; EGF_gRNA

    Plasmid

    #199333

    Purpose

    pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    zCREST3
    Cas9
    nrg1 EGF sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTol2_NBT:Cas9green; erbb2_gRNA171

    Plasmid

    #199337

    Purpose

    pDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    NBT
    Cas9
    erbb2 sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTol2_claudink:Cas9green; control_gRNA

    Plasmid

    #199336

    Purpose

    pDEL143; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous control sgRNA

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Inserts

    claudink
    Cas9
    control sgRNA
    

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET24b-BR1.0

    Plasmid

    #199435

    Purpose

    expresses BAmRed1.0 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMRed
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTYF-mCMV/SYN-EGFP

    Plasmid

    #19975

    Depositor

    Sergey Kasparov

    Article

    Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

    Insert

    mCMV/SYN-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge