We narrowed to 7,808 results for: 199

1. pBMN-HA-TBK1

   Plasmid

   #199205

   Purpose

   Used for the expression of TBK1 (SMC Internal No. 1996)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTP298 His14-Avi-27xGS-SUMOEu-2xGS-anti ALFA tag nanobody

   Plasmid

   #199390

   Purpose

   Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tag

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-ALFA nanobody
   

   Use

   Tags

   14xHis-Avi-SUMOEu

   Expression

   Bacterial

   Mutation

   Promoter

   T5-LacO

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTS117 His14-Avi-45xGS-anti GFP nanobody

   Plasmid

   #199370

   Purpose

   Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   anti-GFP nanobody
   

   Use

   Tags

   14xHis-Avi

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T5-LacO

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKL2351

   Plasmid

   #199721

   Purpose

   A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   NPTII
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-Gm-Pc-tdTomato

   Plasmid

   #199250

   Purpose

   Fluorescent labelling of bacterial cells with tdTomato

   Depositor

   Uwe Mamat

   Article

   Mamat et al Appl Environ Microbiol. 2023 May 17:e003

   Insert

   Codon-optimized tdTomato gene
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimization for Xanthomonadaceae

   Promoter

   Pc promoter from class III integron of Delftia a…

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQIq_MRGS_HIS8_(27F3)_FLAG

   Plasmid

   #199615

   Purpose

   Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27F3
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-LOV-Turbo-NES

   Plasmid

   #199545

   Purpose

   expresses LOV-Turbo in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   NES and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG306-pTet07-nLuc

   Plasmid

   #199755

   Purpose

   Control construct of elongation reporter to quantify elongation of YFP

   Depositor

   Brian Zid

   Article

   Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

   Insert

   Nanoluc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TetO7

   Available since

   May 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-idCas9-KRAB-Hygro donor

   Plasmid

   #199621

   Purpose

   Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

   Depositor

   Danwei Huangfu

   Article

   Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

   Insert

   AAVS1-idCas9-KRAB-Hygro
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCBL101-RUBY

    Plasmid

    #199723

    Purpose

    Expresses betalain biosynthesis genes under CaMV 35S promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    35S-RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKeima-LiveDrop

    Plasmid

    #199695

    Purpose

    lipophagy reporter system based on the Keima-fluorophore

    Depositor

    Tobias Walther

    Article

    Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

    Insert

    GPAT4 (Gpat4 Fly)
    

    Use

    Tags

    Keima

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_CMV::V5-EGFR

    Plasmid

    #199717

    Purpose

    Expresses V5-tagged EGFR in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    V5-EGFR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiGuide Blast

    Plasmid

    #199622

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV_CMV::hTfR-GFP

    Plasmid

    #199714

    Purpose

    Expresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    hTfR-GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX304_CMV::mCherry-LgBiT-FLAG-NES

    Plasmid

    #199715

    Purpose

    Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-LgBiT-FLAG-NES
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTS118 His14-Avi-28xGS-anti ALFA tag nanobody

    Plasmid

    #199371

    Purpose

    Bacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac Dual wt p110α/p85α

    Plasmid

    #199302

    Purpose

    co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

    Depositor

    Peter Vogt

    Article

    Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

    Insert

    His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
    

    Use

    Tags

    Avi and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBroad-T7RNAP-chlorR

    Plasmid

    #199992

    Purpose

    T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorR

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    T7RNAP Circuit
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    copFnull

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF_PE2

    Plasmid

    #199267

    Purpose

    Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

    Plasmid

    #199605

    Purpose

    Expression of cell permeable opAsCas12a in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    opCas12a-T8N
    

    Use

    CRISPR

    Tags

    6xHis, Twin-strep, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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