We narrowed to 2,241 results for: D10

1. pDCC5

   Plasmid

   #59984

   Purpose

   Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

   Depositor

   Peter Duchek

   Article

   Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

   Insert

   Cas9-D10A
   

   Use

   CRISPR

   Tags

   HA

   Expression

   Insect

   Mutation

   D10A

   Promoter

   hsp70Bb

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ265N2

   Plasmid

   #174001

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-UNG for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-SpRY-UNG
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ265O2

   Plasmid

   #174002

   Purpose

   Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1 for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   rAPOBEC1-zCas9(D10A)-SpRY-rXRCC1
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In zCas9(D10A)-SpRY: D10A, A61R, L1111R, D1135L, …

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ265L2

   Plasmid

   #174000

   Purpose

   Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY for C-G base editing

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

   Insert

   UNG-rAPOBEC1(R33A)-zCas9(D10A)-SpRY
   

   Use

   CRISPR

   Tags

   3X FLAG, NLS

   Expression

   Plant

   Mutation

   In rAPOBEC1: R33A; in zCas9(D10A)-SpRY: D10A, A61…

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AIO-Puro

   Plasmid

   #74630

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Puromycin resistant marker

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available Since

   May 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH-ABE8e

   Plasmid

   #200774

   Purpose

   For plant adenine base editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

   Insert

   nCas9(D10A)-TadA8e
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A for Cas9; W23R, H36L, P48A, R51L, L84F, A106…

   Promoter

   maize Ubiquitin-1, OsU3

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH-ABE8e-spG

   Plasmid

   #200775

   Purpose

   For plant adenine base editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

   Insert

   nCas9-spG(D10A)-TadA8e
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

   Promoter

   maize Ubiquitin-1, OsU3

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. miniCGBE1-SpRY (pBM1571)

   Plasmid

   #170105

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

   Promoter

   CMV

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORFE0002

   Plasmid

   #112071

   Purpose

   AtCAS9 nickase D10A module CDS1

   Depositor

   Christian Chevalier

   Insert

   AtCAS9 nickase D10A
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Plant codon optimized nCas9 D10A S. pyogenes

   Promoter

   Available Since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORFE0003

    Plasmid

    #112072

    Purpose

    AtDeadCAS9 D10A H840A module CDS1

    Depositor

    Christian Chevalier

    Insert

    AtDeadCAS9 D10A H840A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pORFE0014

    Plasmid

    #112076

    Purpose

    AtCAS9 nickase D10A module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    AtCAS9 D10A without stop codon
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORFE0005

    Plasmid

    #112073

    Purpose

    AtDeadCAS9 D10A H840A without stop codon module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    AtDeadCAS9 D10A H840A without stop codon
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pITI2

    Plasmid

    #166095

    Purpose

    Gal inducible expression of D10A-Cas9 nickase

    Depositor

    Rebecca Burgess

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A

    Promoter

    Gal-inducible

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPH3

    Plasmid

    #166098

    Purpose

    Gal inducible expression of D10A-Cas9 nickase

    Depositor

    Rebecca Burgess

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A

    Promoter

    Gal-inducible

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-ABE8eWQ

    Plasmid

    #161815

    Purpose

    Expresses ABE8eWQ in mammalian cells

    Depositor

    Sangsu Bae

    Insert

    bpNLS-TadA8e(V106W/D108Q)-32AA linker-hSpCas9(D10A)-bpNLS-P2A-EGFP-NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLCNICK

    Plasmid

    #84653

    Purpose

    Genome editing for Lactobacillus casei Lc2W

    Depositor

    Sheng Yang

    Article

    Song et al Appl Environ Microbiol. 2017 Sep 1. pii:

    Inserts

    Cas9 nickase
    sgRNA
    homology arms of LC2W_2179
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ151

    Plasmid

    #69302

    Purpose

    Gateway entry vector with pcoCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pcoCas9D10A (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ155

    Plasmid

    #69306

    Purpose

    Gateway entry vector with AteCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9D10A (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ159

    Plasmid

    #69308

    Purpose

    Gateway entry vector with hSpCas9D10A

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    hSpCas9D10A (Human codon optimized)
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mutation

    D10A mutation; NLS fusion

    Promoter

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVF28

    Plasmid

    #229469

    Purpose

    Expresses RssB with D104A mutation

    Depositor

    Alexandra Deaconescu

    Article

    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

    Insert

    RssB (D104A)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D104A

    Promoter

    T7

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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