We narrowed to 534 results for: DSE

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pExpreS2-2

Plasmid

#175362

Purpose

G418R backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

Depositor

ExpreS2ion Biotechnologies

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) p65 shRNA1

Plasmid

#22507

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA1 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pJFRC-20X-UAS-ADARcd

Plasmid

#81173

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Article

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

UAS-ADARcd

Expression

Insect

Promoter

UAS

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) p65 shRNA2

Plasmid

#22508

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

p65 shRNA2 (Rela Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
pSicoR (GFP) cRel shRNA1

Plasmid

#22509

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

cRel shRNA1 (Rel Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pSicoR (Puro) cRel shRNA2

Plasmid

#22510

Depositor

Tyler Jacks

Article

Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

Insert

cRel shRNA2 (Rel Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only
SP_G1

Plasmid

#228181

Purpose

Plasmid contains the secretion signal peptide sequence of the yeast DSE2 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

Depositor

Joseph Brock

Article

Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

Insert

sp_P38844_DSE2_YEAST:0-21

Use

Synthetic Biology

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRi (Gen1)

Plasmid

#73497

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB-P2A-mCherry
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRi (Gen2)

Plasmid

#73498

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc[Chronos-GFP]

Plasmid

#62722

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FLEX-rc[Chronos-GFP]

Plasmid

#62725

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-rc [Chronos-tdTomato]

Plasmid

#84484

Purpose

AAV-mediated expression of Chronos-tdTomato under the CAG promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. tdTomato is a codon diversified version.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Promoter

CAG

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc[CoChR-GFP]

Plasmid

#62724

Purpose

AAV-mediated expression of CoChR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

CoChR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

human synapsin promoter

Available Since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

Plasmid

#122102

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-FLPX-rc [Chronos-tdTomato]

Plasmid

#105834

Purpose

AAV-mediated expression of Chronos-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-tdTomato

Use

AAV

Tags

tdTomato (codon diversified version)

Expression

Mammalian

Promoter

CAG

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-Chronos-GFP

Plasmid

#122098

Purpose

AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α (1.1 kb short version)

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS

Plasmid

#85821

Purpose

iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

Zf Ubi::cMyc oDam_f_GFPoNLS

Use

Tol2 based vector

Tags

cMyc N terminal to oDam_f_GFPoNLS and oNLS (optim…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-RbE2Fmut

Plasmid

#32391

Depositor

Julien Sage

Article

Burkhart et al Mol Cell Biol. 2010 Apr;30(7):1729-45. E

Insert

Rb promoter (Rb1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated E2F binding site: TTTCCCGC --> TTTACATC

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA4-Claspin-Flag

Plasmid

#136246

Purpose

Protein expression of Claspin with a C terminal FLAG tag

Depositor

Aziz Sancar

Article

Sar et al J Biol Chem. 2004 Sep 17;279(38):39289-9

Insert

Claspin

Tags

Flag

Expression

Mammalian

Mutation

E263G, Q499R, I783T, D932G and S1062G- Please see…

Available Since

Jan. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-ECC-Biosensor

Plasmid

#140832

Purpose

Encoding the CAG promoter based eccDNA biosensor

Depositor

Yonglun Luo

Article

Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

Insert

ECC-Biosensor

Use

Piggybac (pb) transposon

Expression

Mammalian

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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