Addgene

We narrowed to 1,961 results for: IPP

Showing: 241 - 260 of 1961 results

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mApple-M1 SpastinE442Q

Plasmid

#134464

Purpose

M1 SpastinE442Q expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Glu to Gln at amino acid 442

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pPHR-iRFP713

Plasmid

#103818

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-iRFP713 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinK388R

Plasmid

#134463

Purpose

M1 SpastinK388R expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

Lys to Arg at amino acid 388

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Cerulean-GalT

Plasmid

#11930

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Use

Tags

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Promoter

Available since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98H-pRc/CMV

Plasmid

#22044

Depositor

William Kaelin

Article

Ohh et al Mol Cell. 1998 Jun . 1(7):959-68.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 98 from Tyrosine to H…

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pEAK-cKal7

Plasmid

#145801

Purpose

expresses Kalirin-7 with the c-front in mammalian cells

Depositor

Richard Mains, Betty Eipper

Article

Miller et al J Neurochem. 2017 Mar;140(6):889-902. do

Insert

cKalirin-7 (Kalrn Rat)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

EIF2a

Available since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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mApple-M11-92

Plasmid

#134466

Purpose

M11-92 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin Amino Acids 1-92 (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

M1 Spastin Amino Acids 1-92

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP

Plasmid

#103815

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCIBN-mCherry

Plasmid

#103816

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-NLS

Plasmid

#103819

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-mCherry-NLS (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS

Plasmid

#103820

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-NLS (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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PHR-GFP

Plasmid

#183926

Purpose

Optogenetic PHR domain coupled to GFP; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP (CRY2 Mustard Weed, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP

Plasmid

#103817

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP (CRY2 Mustard Weed)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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SIV_Rev

Plasmid

#236244

Purpose

3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

SIV Rev

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV[FLEX]-CAG-EGFP-pri-miR-137

Plasmid

#235159

Purpose

Expresses EGFP and miR-137 primary transcript in a Cre recombinase-dependent manner

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

EGFP/pri-miR-137 (Mir137 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-rev(CMV-TagBFP2)-EFS-mCherry-124-MUT-MRE

Plasmid

#235148

Purpose

Expresses the mCherry-based mutated (control) sensor for miR-124. TagBFP2 is expressed as a transduction reporter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mCherry with a mutated miR-124 MRE

Use

AAV

Tags

Expression

Mutation

7 nucleotides of the miR-124 WT MRE mutated

Promoter

EFS

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2E

Plasmid

#225072

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2E)

Use

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pJFRC-10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

Plasmid

#217514

Purpose

Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrTdtomato and KD Recombinase

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

10XUAS-FRT-STOP-FRT-myrTdtomato-2A-KDR::Pest

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VP16

Plasmid

#183935

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VP16 (UL48 Synthetic)

Use

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCIS-rPALcc

Plasmid

#145804

Purpose

expresses the catalytic core of rat peptidyl-hydroxyglycine-alpha-amidating lyase in mammalian cells

Depositor

Richard Mains, Betty Eipper

Article

Kolhekar et al Biochemistry. 2002 Oct 15;41(41):12384-9

Insert

rat PALcc (peptidyl-hydroxyglycine alpha-amidating monooxygenase) (Pam Rat)

Use

Tags

Expression

Mammalian

Mutation

changed serine 767 to alanine to prevent glycosyl…

Promoter

CMV

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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