We narrowed to 32,125 results for: PLE

1. pLenti CMV/TO SV40 small + Large T (w612-1)

   Plasmid

   #22298

   Depositor

   Eric Campeau

   Insert

   SV40 small + Large T antigen
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20942

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   EF1alpha

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti CMV/TO GFP-Zeo DEST (719-1)

   Plasmid

   #17431

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Synapsin-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20945

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   hSyn

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

   Plasmid

   #20943

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available since

   July 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

   Plasmid

   #20294

   Purpose

   Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

   Depositor

   Karl Deisseroth

   Article

   Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed Cysteine 128 to Serine

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. hu-TLR4 399 SNP flag pLenti4/To/v5/DEST

   Plasmid

   #42651

   Purpose

   destination vector for lentivirus-mediated expression of human TLR4 399 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 399 SNP (TLR4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   T399I

   Promoter

   CMV/TO

   Available since

   March 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CaMKIIa-hChR2(H134R)-EYFP-WPRE

   Plasmid

   #20944

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

9. hu-TLR4 299 SNP flag pLenti 4/to/v5 pDEST

   Plasmid

   #42650

   Purpose

   destination vector for lentivirus-mediated expression of human TLR4 299 SNP in mammalian cells

   Depositor

   Scott Friedman

   Article

   Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

   Insert

   TLR4 299 SNP (TLR4 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D299G

   Promoter

   CMV/TO

   Available since

   March 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

    Plasmid

    #20293

    Purpose

    Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Alanine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV GSE T329A Hygro (w189-4)

    Plasmid

    #22255

    Depositor

    Eric Campeau

    Insert

    Genetic suppressor element #22, T329A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Threonine 329 is mutated to Alanine (p53 nomencat…

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

    Plasmid

    #20295

    Purpose

    Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Threonine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti CMV GFP Hygro (656-4) (Lentiviral Prep)

    Viral Prep

    #17446-LV

    Purpose

    Ready-to-use Lentiviral Prep particles produced from pLenti CMV GFP Hygro (656-4) (#17446). In addition to the viral particles, you will also receive purified pLenti CMV GFP Hygro (656-4) plasmid DNA. Lentiviral particles carrying the GFP and hygromycin resistance.

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Promoter

    Tags

    None

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

14. Human Lipid Droplet and Metabolism Library

    Pooled Library

    #191535

    Purpose

    Lentiviral CRISPR library targeting 1,196 genes related to lipid droplet biology and cell metabolism.

    Depositor

    James Olzmann

    Article

    Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

    Expression

    Mammalian

    Use

    CRISPR and Lentiviral

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

15. Sabatini/Lander Lab Activity-Optimized Genome-wide Mouse sgRNA Library in pLenti-sgRNA

    Pooled Library

    #1000000096

    Purpose

    CRISPR gRNA knockout pooled library for targeting the mouse genome

    Depositor

    Eric Lander, David Sabatini

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

16. Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA

    Pooled Library

    #1000000095

    Purpose

    CRISPR gRNA knockout pooled library for targeting the human genome

    Depositor

    Eric Lander, David Sabatini

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

17. Multiplex Yeast Toolkit (MYT)

    Plasmid Kit

    #1000000229

    Purpose

    The Multiplex Yeast Toolkit (MYT) is a collection of 96 plasmids for genetically engineering the yeast, Saccharomyces cerevisiae.

    Depositor

    Tom Ellis

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

18. CRISPRainbow Kit for Multiplex Labeling

    Plasmid Kit

    #1000000079

    Purpose

    CRISPR/Cas9 to tag up to 7 different genomic locations. Unique labeling system allows scientists to track DNA movement in live cells and study the structure of the genome in real time.

    Depositor

    Thoru Pederson

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit

    #1000000060

    Purpose

    Multiple Lentiviral Expression (MuLE): modular system used to construct complex lentiviruses for modification of mammalian cells with a single viral infection.

    Depositor

    Ian Frew

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-TripleColour-StAR

    Plasmid

    #222695

    Purpose

    Competition assay

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP, miRFP703, dTomato

    Expression

    Mammalian

    Mutation

    PGK, TagBFP, dTomato without BsmBI cutsite

    Promoter

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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