We narrowed to 32,159 results for: uros

1. pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc

   Plasmid

   #161584

   Purpose

   expression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutation

   Depositor

   Maik Hintze

   Insert

   Lrrk2 (Lrrk2 Mouse)
   

   Tags

   Myc tag and StrepII

   Expression

   Mammalian

   Mutation

   D1994S

   Promoter

   CMV

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

   Plasmid

   #162335

   Purpose

   Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pan-Neurofascin (extracellular) scFv [A12/18]

   Plasmid

   #190485

   Purpose

   Mammalian Expression of Pan-Neurofascin (extracellular) scFV. Derived from hybridoma A12/18.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Pan-Neurofascin (extracellular) (Rattus norvegicus) recombinant scFV (Nfasc Mouse)
   

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

   Plasmid

   #139448

   Purpose

   Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag E2-crimson
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PEA1-Puro-HEK3 CTT ins

   Plasmid

   #171996

   Purpose

   Delivers all prime editing (nickase) components targeting the HEK3 site for a CTT inserrtion, in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA HEK3 +90
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-FHH-NRASQ61K-IRES-Puro

   Plasmid

   #120570

   Purpose

   Expresses Flag-HA-6xHIS-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   Flag-HA-6xHIS

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBRY-nuclear mCherry-IRES-PURO

   Plasmid

   #52409

   Purpose

   Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   nuclear mCherry
   

   Expression

   Mammalian

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSUPER retro puro GFP shRNA

   Plasmid

   #30519

   Depositor

   John Gurdon

   Article

   Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

   Insert

   GFP shRNA
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe 3XFLAG-wtGATA6-3XAU1 puro

   Plasmid

   #72607

   Purpose

   Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   GATA binding protein 6 (GATA6 Human)
   

   Use

   Retroviral

   Tags

   3XAU1 and 3XFLAG

   Expression

   Mammalian

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-TRE3G-BE3RA-PGK-Puro

    Plasmid

    #110846

    Purpose

    Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA
    

    Use

    Lentiviral

    Mutation

    D10A

    Promoter

    TRE3G

    Available Since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEpicVector3mC_Puro-T2A-mCherry-VSVg-HA-AU1

    Plasmid

    #162107

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to VSVg-HA-AU1
    

    Use

    Lentiviral

    Tags

    VSVg-HA-AU1

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-HA-FLAG

    Plasmid

    #162112

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-HA-FLAG
    

    Use

    Lentiviral

    Tags

    StrepTagII-HA-FLAG

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-HA

    Plasmid

    #162089

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to StrepTagII-ProtC-HA
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-HA

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEpicVector3G_Puro-T2A-EGFP-VSVg-StrepTagII-HA

    Plasmid

    #162080

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-StrepTagII-HA
    

    Use

    Lentiviral

    Tags

    VSVg-StrepTagII-HA

    Mutation

    WT

    Promoter

    EF1A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1119 pBabe puroL HA FKHR AAA

    Plasmid

    #9025

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pBABE puro human RXR alpha

    Plasmid

    #11441

    Depositor

    Ronald Kahn

    Insert

    RXR alpha (RXRA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pSBbi-moxGFP-P2A-T2A-Puro

    Plasmid

    #176893

    Purpose

    Sleeping Beauty transposon-based vector for mammalian polycistronic expression of moxGFP and two additional genes of interest

    Depositor

    Jorge Luis Arias-Arias

    Article

    Acon et al iScience. 2021 Nov 8;24(12):103407. doi:

    Insert

    moxGFP-P2A-T2A
    

    Use

    Transposon

    Tags

    moxGFP

    Expression

    Mammalian

    Promoter

    EF1a/RPBSA

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPaint-mNeon-F-mgp100-PuroR [M1G]

    Plasmid

    #171802

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, murine gp100 CD8+ T cell epitope [AA: 25-33 (EGSRNQDWL)] and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-FLAG-mgp100-T2A-PuroR
    

    Use

    Gene tagging

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-puro/Flag PD-L1 N192Q/N200Q

    Plasmid

    #121453

    Purpose

    expression of human PD-L1 mutant in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    CD274 (CD274 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N192Q/N219Q

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA4TO-CAMSAP1L1-24xGCN4-IRES-puro

    Plasmid

    #60912

    Purpose

    Enocdes Camsap1L1 fused to the SunTag for single molecule imaging

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

    Insert

    Camsap1L1
    

    Expression

    Mammalian

    Available Since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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