167,433 results

1. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC9A3

   Plasmid

   #132234

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC9A3 (SLC9A3 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCDH-EF1a-mCherry-EGFP-LC3B

   Plasmid

   #170446

   Purpose

   Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   mCherry-GFP-LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFD152

   Plasmid

   #125546

   Purpose

   derivative of pFD116 with an optimized RBS for E. coli

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Insert

   optimized RBS
   

   Use

   CRISPR

   Promoter

   anhydrotetracycline Ptet promoter

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA GSK3 beta wt pcDNA3

   Plasmid

   #14753

   Purpose

   Mammalian expression of human GSK3 beta with HA tag

   Depositor

   Jim Woodgett

   Article

   He et al Nature. 1995 Apr 13. 374(6523):617-22.

   Insert

   GSK3 beta (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV1)

   Viral Prep

   #100853-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Promoter

   Syn

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Mutation

   DR1 A>T

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-GFP (AAV8)

   Viral Prep

   #37825-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Edward Boyden

   Promoter

   CAG

   Tags

   GFP

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

9. rtTA-N144

   Plasmid

   #66810

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

   Depositor

   Andrew Yoo

   Article

   Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

   Insert

   Reverse tetracycline-controlled transactivator
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human EF1a

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-DIO-mCherry

    Plasmid

    #50459

    Purpose

    Double floxed mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Promoter

    human Synapsin 1

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-CAG-GFP

    Plasmid

    #28014

    Depositor

    Karel Svoboda

    Article

    Mao et al Neuron. 2011 Oct 6;72(1):111-23.

    Insert

    eGFP
    

    Use

    AAV; Adeno associate viral

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-EGFP-KASH

    Plasmid

    #154373

    Purpose

    Vector for Cre-dependent expression of EGFP tagged KASH protein domain

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    EGFP-KASH
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-HA-PD-L1

    Plasmid

    #121492

    Purpose

    Expression of human PD-L1 WT in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 (CD274 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. Red cGull

    Plasmid

    #191853

    Purpose

    Expresses biosensor for cGMP in mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

    Insert

    Red cGull
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAVS1-P-MCS

    Plasmid

    #80488

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hsyn-GRAB_rDA1h (AAV9)

    Viral Prep

    #140557-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_rDA1h (#140557). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_rDA1h plasmid DNA. Synapsin-driven expression of red fluorescent, high affinity dopamine sensor GRAB_rDA1h. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Promoter

    Syn

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

17. pRK7-HA-S6K1-KR

    Plasmid

    #8985

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K100R, kinase-inactive

    Available Since

    Jan. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET Nucleolin

    Plasmid

    #13037

    Depositor

    Peter Newburger

    Article

    Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

    Insert

    Nucleolin (NCL Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVITRO1-Trastuzumab-IgG1/κ

    Plasmid

    #61883

    Purpose

    Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    mEF1 Prom and rEF1 Prom

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV5)

    Viral Prep

    #112677-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Promoter

    EF1a

    Tags

    mCherry (Cre-negative cells), EGFP (Cre-positive cells)

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only