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Showing: 5281 - 5320 of 13166 results

  3. pRhaCAST

    Plasmid
    #211791
    Purpose
    pZLrhaB2plus with Sh-cas12K-tnsBC-tniQ cloned into, which drives by the rhamnose inducible promoter
    Depositor
    Silvia Cardona
    Article
    Yap et al Appl Environ Microbiol. 2024 Jul 24;90(7
    Insert
    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Rhamnose-inducible
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    211791-plasmid-map-sequence-id-418380

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  4. pITEdv1

    Plasmid
    #218283
    Purpose
    Use together with with pJE13HD7 and pJE13HD7 derivatives to integrate heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    Hph>tAgTEF1-ISceI-Repeat1-HO(1828, 1979)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218283-plasmid-map-sequence-id-433685

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  5. pITEu2

    Plasmid
    #218282
    Purpose
    Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218282-plasmid-map-sequence-id-433684

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  6. pJE13HD7

    Plasmid
    #218281
    Purpose
    Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.
    Depositor
    Claudia Vickers
    Article
    Evans et al bioRxiv 2024.01.30.578080
    Insert
    HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218281-plasmid-map-sequence-id-433683

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  7. SUMO CRYGS 16-177 Q16C

    Plasmid
    #217673
    Purpose
    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Use
    Tags
    6XHis and SUMO
    Expression
    Bacterial
    Mutation
    Glutamine 16 changed to Cysteine, and amino acidsā€¦
    Promoter
    T7
    Available since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217673-plasmid-map-sequence-id-433254

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  13. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Expression
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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  31. MLKS2delARMec

    Plasmid
    #130860
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Gateway entry vector
    Tags
    eGFP-FLAG-HA
    Expression
    Mutation
    Deletion of armadillo domain
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130860-plasmid-map-sequence-id-255666

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  32. MLKS2del4exp

    Plasmid
    #130861
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of terminal four amino acids
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130861-plasmid-map-sequence-id-255913

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  33. MLKS2delKASHexp

    Plasmid
    #130862
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of KASH domain
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130862-plasmid-map-sequence-id-255915

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  34. MLKS2delTMexp

    Plasmid
    #130863
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of transmembrane domain
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130863-plasmid-map-sequence-id-255916

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  35. MLKS2delARMexp

    Plasmid
    #130864
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of armadillo domain
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130864-plasmid-map-sequence-id-255668

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  36. MLKP2delKASH

    Plasmid
    #130838
    Purpose
    Expression of plant LINC fluorescent fusion protein for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs
    Insert
    MLKP2
    Use
    Tags
    eGFP-FLAG-HA
    Expression
    Plant
    Mutation
    Deletion of KASH domain, addition of three alaniā€¦
    Promoter
    CaMV 35S
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130838-plasmid-map-sequence-id-255274

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  37. MLKS2del4ec

    Plasmid
    #130857
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Gateway entry vector
    Tags
    eGFP-FLAG-HA
    Expression
    Mutation
    Deletion of terminal four amino acids
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130857-plasmid-map-sequence-id-255655

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  38. MLKS2delKASHec

    Plasmid
    #130858
    Purpose
    Expression of plant nuclear envelope LINC protein fused to fluorescent protein and affinity tags for cell biology
    Depositor
    Hank Bass
    Article
    Gumber et al Nucleus. 2019 Dec;10(1):144-166. doi: 10
    Insert
    MLKS2
    Use
    Gateway entry vector
    Tags
    eGFP-FLAG-HA
    Expression
    Mutation
    eGFP-FLAG-HA
    Promoter
    Available since
    Sept. 28, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    130858-plasmid-map-sequence-id-255656

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  39. pJAT58

    Plasmid
    #204304
    Purpose
    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    3XP3-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    3XP3
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204304-plasmid-map-sequence-id-406184

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  40. pJAT71

    Plasmid
    #204305
    Purpose
    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable ie1XP3-DsRed cassette expressed in abdomen and mouthparts.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    ie1-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ie1
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    204305-plasmid-map-sequence-id-405846

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Showing: 5281 - 5320 of 13166 results