We narrowed to 23,247 results for: crispr

1. pXPR_011

   Plasmid

   #59702

   Purpose

   This lentiviral vector can be used to assay Cas9 activity.

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pnCasPA-BEC

   Plasmid

   #113349

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in Pseudomonas species

   Depositor

   Quanjiang Ji

   Article

   Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFYF1327 EGFP Site#2

   Plasmid

   #47512

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 2
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #50946

   Purpose

   An empty gRNA expression vector

   Depositor

   Kosuke Yusa

   Article

   Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-SMVP-Cas9N

   Plasmid

   #80930

   Purpose

   Expresses Cas9N in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330A-FBL/PITCh

    Plasmid

    #63671

    Purpose

    Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

11. SaCas9

    Plasmid

    #188494

    Purpose

    Expresses FLAG tagged SaCas9

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. SpdCas9 VPR

    Plasmid

    #188509

    Purpose

    Expresses FLAG tagged SpdCas9 VPR

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AsCas12a

    Plasmid

    #188492

    Purpose

    Expresses FLAG tagged AsCas12a

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    Cas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQAT05

    Plasmid

    #223377

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-Puro (PX459)

    Plasmid

    #48139

    Purpose

    NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pEn-Chimera

    Plasmid

    #61432

    Purpose

    Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ141B2.0

    Plasmid

    #99897

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ141D2.0

    Plasmid

    #99906

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2CT-His-MBP-Lbu_C2c2_WT

    Plasmid

    #83482

    Purpose

    Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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