We narrowed to 13,838 results for: CRISPR-Cas9

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pME-nCas9n-P2A-mKate2-pA

Plasmid

#109547

Purpose

Multisite gateway vector for expression of nCas9n with bicistronic expression of mKate2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nCas9n and mKate2

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9HF1-P2A-puro

Plasmid

#214357

Purpose

Expresses SpCas9HF1 and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SpCas9HF1 and puromycin resistance genes

Expression

Mammalian

Mutation

N497A/R661A/Q695A/Q926A

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-CfANLN_sgRNA

Plasmid

#183879

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

Canis familiaris ANLN sgRNA spacer

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSaCas9-sgRNA-Tetra-com vector

Plasmid

#131227

Purpose

For expressing gRNA, whose Tetraloop is replaced with a com aptamer

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

vector for com modified sgRNA

Use

AAV

Expression

Mammalian

Available Since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-dCas9-KRAB-gRNA-TRE-blast

Plasmid

#201152

Purpose

Lentiviral expression of S. pyogenes dead Cas9 (dCas9/dSpCas9/SpdCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

Depositor

Moshe Szyf

Inserts

dCas9-KRAB
gRNA-TRE

Use

CRISPR and Lentiviral

Tags

HA

Mutation

gRNA sequence: TACGTTCTCTATCACTGAT

Available Since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

Plasmid

#172982

Purpose

control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

control sgRNA

Use

CRISPR and Lentiviral

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

Plasmid

#186745

Purpose

dCas9-10XGcn4-P2A-mCherry expression construct

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

Cas9-10XGcn4-P2A-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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dCas9 gateway compatible

Plasmid

#128322

Purpose

dCas9 C-terminal gateway cloning vector

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9 gateway compatible

Plasmid

#128321

Purpose

Cas9 C-terminal gateway fusion

Depositor

Alejandro Chavez

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-sgACSL3.C-Cas9-P2A-Puro

Plasmid

#129412

Purpose

Encoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminus

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

SpCas9 and sgACSL3.C (ACSL3 Human)

Use

CRISPR

Tags

P2A-Puro

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pINDUCER dCas9-TET1CD

Plasmid

#101921

Purpose

The dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).

Depositor

Danwei Huangfu

Article

Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

Insert

dCas9-TET1CD

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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2333_Cas9-CtIP-dnRNF168

Plasmid

#200254

Purpose

This plasmid expresses the Cas9-CtIP-dnRNF168 in mammalian cells

Depositor

Claudio Mussolino

Article

Carusillo et al Nucleic Acids Res. 2023 May 22;51(9):466

Insert

Cas9-CtIP-dnRNF168

Expression

Mammalian

Promoter

CMV

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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Fuw-dCas9-Dnmt3a

Plasmid

#84476

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Synthetic, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

Plasmid

#172985

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D

Plasmid

#110302

Purpose

Expresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAWPSG-Ptet-nCas9-BE

Plasmid

#195739

Purpose

Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

Depositor

Seung-Goo Lee

Article

Jeong et al Chem Eng J, 2023

Insert

Tet repressor protein / APOBEC1-nCas9(D10A)-UGI

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Ptet(Tetracycline inducible protein)

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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U6-CjCas9-MS2gRNA-Backbone

Plasmid

#210711

Purpose

This plasmid express MS2 loop containing Cj Cas9 gRNA

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MS2 loop containing Cj Cas9 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#2

Plasmid

#172984

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCas9 PE2*-3HA

Plasmid

#169854

Purpose

Mammalian Expression, SpyCas9 PE2* prime editor with 3HA-tag

Depositor

Scot Wolfe

Article

Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

Insert

SpCas9-H840A-star-M-MLV-3HA

Tags

3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…

Expression

Mammalian

Promoter

cmv

Available Since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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