We narrowed to 43,229 results for: cha

1. Kv7.5 AB (361-545, Δ395-511)/pET28HMT

   Plasmid

   #111267

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.5 AB (361-545, Δ395-511)

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1

   Plasmid

   #111460

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/I346A, C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1

   Plasmid

   #111461

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/I346D, C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Kv7.4 (1-645, Δ368-523)/S536A/L536A/HA tag/pcDNA3.1

   Plasmid

   #111462

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/S536A/L540A, C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Kv7.4 (1-645, Δ368-523)/S536D/L540D/HA tag/pcDNA3.1

   Plasmid

   #111497

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523)/S536D/L540D, C643A

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. rAAV2-retro helper

   Plasmid

   #81070

   Purpose

   retrograde AAV helper vector, carrying rep and modified capsid gene

   Depositor

   Alla Karpova, David Schaffer

   Article

   Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

   Inserts

   rep
   rAAV2-retro cap
   

   Use

   AAV and Unspecified; Helper vector

   Tags

   Expression

   Mutation

   Pro2Ala, possibly Asp613Gly and changes made to A…

   Promoter

   Available since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5 FRT/TO SF-HERC2 (ShB-R)

   Plasmid

   #55613

   Purpose

   Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (WT) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Silent mutations have been incorporated into the …

   Promoter

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. U6-hGRIN2B-CAG-ps-SpCas9

   Plasmid

   #102851

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Synthetic, Human, S. pyogenes)
   

   Use

   CRISPR

   Tags

   3X Flag and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. his3MX6-ins3

   Plasmid

   #195040

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   His5+
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. U6-hGRIN2B-CAG-ps-SaCas9

    Plasmid

    #102853

    Purpose

    A single-chain light-controllable dSaCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (GRIN2B Synthetic, S. aureus, Human)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter;

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

    Plasmid

    #153441

    Purpose

    E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc. XDocB binding mode B knock-out mutant
    

    Use

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Arginine 140 and methionine 144 were mutated to a…

    Promoter

    T7 promoter

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

    Plasmid

    #111398

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

    Plasmid

    #111399

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

    Plasmid

    #111400

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

    Plasmid

    #111451

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PX458-3xHA-FnCas9

    Plasmid

    #130969

    Purpose

    3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    FnCas9
    

    Use

    CRISPR

    Tags

    3xHA-NLS and NLS-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458-3xHA-en31FnCas9ABEmax8.17d

    Plasmid

    #201956

    Purpose

    Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDF-His-mTET1CD∆cat

    Plasmid

    #81054

    Purpose

    bacterial expression of catalytic dead catalytic domain of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    catalytic domain amino acid 1367-2057 with histid…

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid

    #198569

    Purpose

    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    changed glutamic acid E164 to lysine (E164K)

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PX458-3xHA-SpCas9

    Plasmid

    #130968

    Purpose

    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    3xHA-NLS-SpCas9
    

    Use

    CRISPR

    Tags

    3x HA, EGFP, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge