We narrowed to 3,367 results for: Library

1. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138150

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Mutation

   DR1 A>T

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti_gRNA -hMLHdn-Puro

   Plasmid

   #191105

   Purpose

   Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

   Depositor

   Hyongbum Kim

   Article

   Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

   Insert

   hMLH1dn
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   I31 silent mutation (ATA to ATC) to remove early …

   Promoter

   EF-1a

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Available Since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

   Plasmid

   #223175

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Mutation

   DR1 A>T

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. hSTARR-seq_ORI vector

   Plasmid

   #99296

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Expression

   Mammalian

   Available Since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. hSTARR-seq_SCP1 vector_blocking 4

   Plasmid

   #99319

   Purpose

   Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Type

   Empty backbone

   Use

   Hstarr-seq screening vector

   Expression

   Mammalian

   Available Since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET30a-Tn5

   Plasmid

   #127917

   Purpose

   Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

   Depositor

   Silin Zhong

   Article

   Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

   Insert

   Tn5
   

   Tags

   Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   T7

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGPA1

   Plasmid

   #115476

   Purpose

   Generation of mariner-based transposon mutant libraries in Enterococcus faecium

   Depositor

   Willem van Schaik

   Article

   Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Perturb-FISH

   Plasmid

   #220626

   Purpose

   expression of gRNAs under U6T7 promoter

   Depositor

   Samouil Farhi

   Article

   Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   BsmBI

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCKC014

    Plasmid

    #226623

    Purpose

    pLenti wt hTdT template for library 3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    Terminal deoxynucleotidyl transferase (DNTT Human)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. hSTARR-seq_SCP1 vector

    Plasmid

    #99292

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1352wUV2 F2bbs kan GCANNNAGGk

    Plasmid

    #18752

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2008 Jun . 26(6):695-701

    Insert

    3 tandem zinc fingers
    

    Expression

    Bacterial

    Mutation

    A Kan cassette (1090bp) is inserted between two b…

    Available Since

    July 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. Arp2_pLib

    Plasmid

    #173676

    Purpose

    Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    Arp2 (ACTR2 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5A0-T

    Plasmid

    #92016

    Purpose

    Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Promoter

    J23102 Promoter (from Anderson promoter library) …

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSTARR-seq_SCP1 vector_blocking 3

    Plasmid

    #99318

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. IBMc326

    Plasmid

    #161949

    Purpose

    Contains Type IIS restriction site free elements for expression vectors in IBM. Expression vector to generate ORF insertion library in IBM on ECF20

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20(1-5)-BsaI-BsaI-ECF20(187-193)-Ter
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSTARR-seq_OCT4 3UTR vector

    Plasmid

    #99295

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hSTARR-seq_mCP vector

    Plasmid

    #99291

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Arp3_pLib

    Plasmid

    #173677

    Purpose

    Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    Arp3 (ACTR3 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. hSTARR-seq_SCP1 vector_blocking 1

    Plasmid

    #99316

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Expression

    Mammalian

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge