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mEmerald-C-Src-7

Plasmid

#54052

Purpose

Localization: Membrane/Vesicles, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Insert

C-Src

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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Pdar

Plasmid

#24786

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Pdar

Tags

His

Expression

Bacterial

Available Since

Aug. 4, 2011

Availability

Academic Institutions and Nonprofits only
pET30a-SRAP

Plasmid

#84524

Purpose

Expresses murine SRAP domain (250 aa) in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

SRAP domain (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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RhoU

Plasmid

#55698

Purpose

expression of mouse RhoU

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoU (Rhou Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
Prb

Plasmid

#24785

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
Pdar(Asn34Asp)

Plasmid

#24834

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Pdar Asn34Asp

Tags

HA and Myc

Expression

Mammalian

Mutation

Asn34Asp

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only
Prb(Asp83Asn)

Plasmid

#24833

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb(Asp83Asn)

Tags

Avi and His

Expression

Bacterial

Mutation

Asp83Asn

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)

Plasmid

#87203

Purpose

To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 108-130 are inserted.

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreenF

Plasmid

#78181

Purpose

Expresses rsGreenF in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreenF

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)

Plasmid

#87202

Purpose

To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-107 are inserted.

Promoter

CMV

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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RCAS (A) goosecoid (CT#19)

Plasmid

#13984

Depositor

Cliff Tabin

Article

Heanue et al Mech Dev. 1997 Dec . 69(1-2):31-7.

Insert

goosecoid (GSC Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pCLC3sFlag

Plasmid

#52422

Purpose

mammalian expression of flag tagged rat CLC3 short transcript

Depositor

Steven Weinman

Article

Shimada et al Am J Physiol Gastrointest Liver Physiol.

Insert

CLC3 (Clcn3 Rat)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFUPI

Plasmid

#80279

Purpose

A bicistronic lentiviral vector used to stably express cDNAs.

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

Puro resistance cassette, IRES, cloning site

Use

Lentiviral

Expression

Mammalian

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pVH028

Plasmid

#80393

Purpose

Contains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

Taz chimeric histine kinase
CusS (cusS )
NahR (nahR )

Use

Synthetic Biology

Tags

Fused by GS linkers to 4 SH3 domains

Expression

Bacterial

Mutation

Glycine 448 changed to Alanine, makes it primaril…

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-PQBP1

Plasmid

#16174

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Polyglutamine binding protein 1 (PQBP1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pFLAG-4EHP(K121/130/134/222R)

Plasmid

#17353

Depositor

Dong-Er Zhang

Article

Okumura et al Genes Dev. 2007 Feb 1. 21(3):255-60.

Insert

4EHP (EIF4E2 Human)

Tags

Flag

Expression

Mammalian

Mutation

K121/130/134/222R

Available Since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only
pFUPI AoT5Cyp

Plasmid

#80280

Purpose

Expresses Aotus TRIM5 CypA fusion

Depositor

Jeremy Luban

Article

Neagu et al J Clin Invest. 2009 Oct;119(10):3035-47.

Insert

TRIM5 CypA fusion

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)MBII-85-3'ss mut

Plasmid

#67644

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron sorounded by natural exons. To improve snoRNA expression efficiency 3' splice site was mutated to consensus sequences.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Mutation

3' splice site muattion

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PdarC1

Plasmid

#24835

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PdarC1

Tags

HA and Myc

Expression

Yeast

Available Since

Aug. 23, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PrbC10

Plasmid

#24788

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Ile75Val, Asp83Asn, Leu122Pro, and Glu135Lys

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Neo-Bam EB-1

Plasmid

#16474

Depositor

Bert Vogelstein

Article

Su et al Cancer Res. 1995 Jul 15. 55(14):2972-7.

Insert

EB-1 (MAPRE1 Human)

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
NKIRAS2

Plasmid

#55676

Purpose

expression of mouse NKIRAS2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

NKIRAS2 (Nkiras2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
NKIRAS1

Plasmid

#55675

Purpose

expression of mouse NKIRAS1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

NKIRAS1 (Nkiras1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
PH1109

Plasmid

#24830

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Tags

His

Expression

Bacterial

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)

Plasmid

#86922

Purpose

To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-157 is deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pVH009

Plasmid

#80399

Purpose

Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

Depositor

Richard Murray

Article

Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

Inserts

3xFLAG-Venus YFP-AAV
CusR (cusR )
AraC (araC )

Use

Synthetic Biology

Tags

C-terminal LZx domain and N-terminal 3xFLAG tag, …

Expression

Bacterial

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETb-rsGreen0.9

Plasmid

#78179

Purpose

Expresses-rsGreen0.9 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.9

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETb-rsGreen0.7b

Plasmid

#78178

Purpose

Expresses rsGreen0.7b in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7b

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MS4A1 RFP (805)

Plasmid

#62009

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

MS4A1

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pSG5-(nic)p85

Plasmid

#55720

Purpose

eukaryotic expression of both SH2 and the inter-SH2 domain of murine p85α.

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p85α

Expression

Mammalian

Mutation

SH2 and the inter-SH2 domain

Promoter

T7

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
human CRKL transgenic construct

Plasmid

#38186

Depositor

Nora Heisterkamp

Article

Hemmeryckx et al Cancer Res. 2001 Feb 15;61(4):1398-405.

Insert

CRKL (CRKL Human)

Use

Mouse transgenic

Mutation

Contains human CRKL introns

Promoter

human CRKL

Available Since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only
sankE3_5

Plasmid

#24893

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

sank E3_5

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
PrbC5

Plasmid

#24787

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Prb

Tags

His

Expression

Bacterial

Mutation

Asp11Gly, Asp83Asn, Val92Ala, Glu119Gly, and Glu1…

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pBB535

Plasmid

#27392

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ (dnaJ, dnaK E. coli)

Expression

Bacterial

Available Since

March 28, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR-AmTrac

Plasmid

#47770

Purpose

expresses AmTrac in yeast

Depositor

Wolf Frommer

Article

De Michele et al eLife. 2013 Jul 2;2:e00800.

Insert

AmTrac

Tags

mcpGFP

Expression

Yeast

Promoter

PMA

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-luxNeon

Plasmid

#229947

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxNeon

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-luxOFP

Plasmid

#229950

Purpose

Mammalian expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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