We narrowed to 34,877 results for: CaS;

1. BICstim-Gag-dCAS9-VPR

   Plasmid

   #120922

   Purpose

   encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

   Depositor

   Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

   Article

   Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

   Insert

   GAG (FMLV)-dCAS9-VPR
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   hCMV

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-fFuCas9-HTBNLS-hph

   Plasmid

   #121092

   Purpose

   Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

   Depositor

   Xiao-Jun Ji

   Article

   Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

   Insert

   Cas9 and hygromycin resist gene
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   gpdA;trpC

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

   Plasmid

   #122333

   Purpose

   Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   sgRNA for mouse Dnmt3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-dCas13-inactive M3nls

   Plasmid

   #157854

   Purpose

   Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

   Depositor

   David Liu

   Article

   Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

   Insert

   dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   PspCas13b Δ984-1090 H133A; METTL3 D395A

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZLCv2-3xFLAG-dCas9-HA-2xNLS

   Plasmid

   #106357

   Purpose

   Empty backbone for CRISPR/Cas9 engineered chromatin immunoprecipitation

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-SaCas9-U6-sgGrin1

   Plasmid

   #124852

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-dCas9-MQ1(WT)-EGFP

   Plasmid

   #89633

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG and 6*His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB[vasa-Cas9 PUb-ZsGreen]

   Plasmid

   #169012

   Purpose

   For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   Cas9
   

   Use

   Piggybac

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila melanogaster vasa

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-CibN-dCas9-CibN

   Plasmid

   #60553

   Purpose

   Expresses CibN-dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available since

   Feb. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

    Plasmid

    #122334

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAB1620 hU6-BpiI-Cas13bt3-DR

    Plasmid

    #176315

    Purpose

    hU6-BpiI-Cas13bt3-DR (crRNA expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    Cas13bt3 crRNA direct repeat
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. P3-Lenti-dCas9-Tet1-GFP

    Plasmid

    #190729

    Purpose

    Lentiviral construct to express spdCas9-Tet1-GFP.

    Depositor

    Maja Jagodic

    Article

    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

    Insert

    dCas9-tet1
    

    Use

    CRISPR

    Tags

    SV40 NLS and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Pro434 codon from CCG to CCC and Gly468 f…

    Promoter

    UbC

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDuBir-Lbu-dCas13a-avitag

    Plasmid

    #100817

    Purpose

    Dual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13a

    Depositor

    Mitchell O'Connell

    Article

    Tambe et al Cell Rep. 2018 Jul 24;24(4):1025-1036. d

    Insert

    Lbu_Cas13a_R472A_H477A_R1048A_H1053A
    

    Use

    Tags

    AviTag and His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    T7

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ-ABE-Cas9n-Act3.0

    Plasmid

    #178956

    Purpose

    It consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    ABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Pinducer20-dCas9-SunTag-2A-BFP

    Plasmid

    #115779

    Purpose

    targeted DNA methylation tool

    Depositor

    Margaret Goodell

    Article

    Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

    Insert

    dCas9-SunTag-2A-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-opto-hCaspase-4 dCARD

    Plasmid

    #208785

    Purpose

    Inducibly expresses human Caspase 4 lacking the CARD domain, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry Caspase4 dCARD (CASP4 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    CARD domain is removed

    Promoter

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid

    #190598

    Purpose

    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    mCherry - separated by T2A

    Expression

    Mutation

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-dCas9-VP64-BFP

    Plasmid

    #196711

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-VP64-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pspCas9-HF-tetra-com-vector

    Plasmid

    #132555

    Purpose

    Vector plasmid expressing high fidelity spCas9 (R691A) and sgRNA scaffold with com replacing the Tetraloop.

    Depositor

    Baisong Lu

    Article

    Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

    Inserts

    High fidelity Cas9
    sgRNA scaffold with com replacing the Tetraloop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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