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pCMV HA hRB delta CDK + S788

Plasmid

#58916

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S788 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S788 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T356

Plasmid

#58911

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T356 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T356 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T821

Plasmid

#58920

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T821 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T821 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFP-GFP-FLARE-AKAR

Plasmid

#123332

Purpose

Green-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

GFP-GFP-FLARE-AKAR

Tags

6xHIS, EGFP, T7 tag (gene 10 leader), and Xpress …

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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MLS-mir-17-92-Mut92

Plasmid

#64094

Purpose

expresses a mutated version of mir-17-92 (mutation of miR-92 seed)

Depositor

Lin He

Article

Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

Insert

miR-17-92 (MIR17HG Human)

Expression

Mammalian

Mutation

mutation of miR-92 seed sequence

Promoter

MSCV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only
pCMV HA hRB delta CDK + T826

Plasmid

#58921

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T826 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with T826 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
Sema4c-AP-His

Plasmid

#72030

Purpose

Expresses the extracellular region of the Sema4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4c (Sema4c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pLNCX2-mCherry-CHMP2A-siRNAres

Plasmid

#115332

Purpose

expresses siRNA-resistant mCherry-CHMP2A in mammalian cells

Depositor

Sanford Simon

Article

Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

Insert

CHMP2A (CHMP2A Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

mutated to be resistant to siRNA knockdown

Promoter

CMV

Available Since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Sema4c-Fc-His

Plasmid

#72156

Purpose

Expresses the extracellular region of the Sema4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema4c (Sema4c Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Sema6b(L)-Fc-His

Plasmid

#72165

Purpose

Expresses the extracellular region of the Sema6B protein (entire extracellular domain; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6b (Sema6b Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV HA hRB delta CDK + S249

Plasmid

#58908

Purpose

mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S249 restored

Depositor

Steven Dowdy

Article

Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

Insert

Rb (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

delta CDK* with S249 restored

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
Sema3f(L,+)-Fc-His

Plasmid

#72147

Purpose

Expresses the Sema3F protein (truncated at cleavage site P3; ie, long and contains no deletion in exon 3), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3f (Sema3f Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3a(L, del3)-AP-His

Plasmid

#72010

Purpose

Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 3), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Dscam1_7.27.30 EC10-Fc-His

Plasmid

#72190

Purpose

Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Dscam1_7.27.30

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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Ncam1_1.0.1-AP-His

Plasmid

#71961

Purpose

Expresses the extracellular region of the NCAM1, isoform 1.0.1 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ncam1_1.0.1 (Ncam1 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Sema3a(S)-AP-His

Plasmid

#72012

Purpose

Expresses the Sema3A protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3a (Sema3a Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-Venus-FLARE-AKAR

Plasmid

#123331

Purpose

Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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Sema3b(L)-AP-His

Plasmid

#72013

Purpose

Expresses the Sema3B protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3b (Sema3b Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMT-GDNF

Plasmid

#119897

Purpose

Mouse GDNF In situ hybridization probe

Depositor

Wenqin Luo

Article

Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

Insert

glial cell line derived neurotrophic factor (Gdnf Mouse)

Use

In situ hybridization probe

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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His10-B1 Integrin

Plasmid

#186139

Purpose

For expression of recombinant his10-beta1 integrin cytosolic domain in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

ITGB1 (ITGB1 Human)

Tags

MBP + uncleavable His10

Expression

Bacterial

Promoter

Lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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