We narrowed to 9,855 results for: control

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RCAN1

Plasmid

#65413

Purpose

Full length RCAN1 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1 (Rcan1 Mouse)

Tags

Flag tagged

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pTet-On-TAP

Plasmid

#59017

Purpose

Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.

Depositor

David Sibley

Article

Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

Type

Empty backbone

Use

Toxoplasma expression

Tags

Calmodulin Binding Peptide fused with a TEV Cleav…

Promoter

RPS13 with Tet Operators Inserted

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pT2ADW-4146NES(ROCK)

Plasmid

#162485

Purpose

For generating transgenic mice expressing ROCK biosensor based on FRET.

Depositor

Michiyuki Matsuda

Article

Imanishi et al Am J Pathol. 2020 Oct 15. pii: S0002-944

Insert

ROCK biosensor based on FRET.

Tags

YPet and ECFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-NLuc-MBP-6xHis

Plasmid

#135931

Purpose

Expresses NanoLuciferase in bacterial cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

MBP-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pBSV2G_P0826-BB0323-mCherryBb

Plasmid

#118244

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

BB0323-mCherry translational fusion under the control of the bb0826 promoter

Tags

mCherry

Expression

Bacterial

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRN3P-HA-Suv4-20h2

Plasmid

#86691

Purpose

For transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)

Tags

HA

Promoter

T3

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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rs214-minor

Plasmid

#113380

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs2149943 minor allele (T)

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs2149943 minor allele

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHES830

Plasmid

#87944

Purpose

Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

Zif268 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)

Expression

Yeast

Promoter

ADH1 (crippled)

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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EcNusGFL

Plasmid

#113122

Purpose

Expresses full-length E. coli NusG

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

NusG (nusG Escherichia coli)

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RVH)-Bre1

Plasmid

#116885

Purpose

FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations

Depositor

Brian Kuhlman

Article

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RVH)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pA5c-tesA-vhb50-8fadR

Plasmid

#86601

Purpose

expression of tesA and Vhb50 under the control of a LacUV5 promoter and fadR under the control of a pBAD promoter

Depositor

Yinjie Tang

Article

Liu et al Biotechnol Bioeng. 2016 Aug 6. doi: 10.1

Inserts

tesA
Vhb50
fadR

Expression

Bacterial

Promoter

LacUV5 and pBAD

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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ER50-SpCas9-ER50

Plasmid

#85448

Purpose

Expresses SpCas9 fused to ER50 domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

ER50-SpCas9-ER50

Use

AAV

Tags

destabilized domain of estrogen receptor ligand b…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LINXa4(RLR)-Bre1

Plasmid

#116884

Purpose

FLAG-tagged LINXa4-Bre1 with RLR hinge-loop mutations

Depositor

Brian Kuhlman

Article

Lerner et al ACS Synth Biol. 2018 Nov 29. doi: 10.102

Insert

LINXa4(RLR)-Bre1

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Bre1 point mutations K8A, K9A and K11A, LINXa3 wi…

Promoter

ADH1

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)-pA

Plasmid

#100278

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain active form.

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Mutation

catalytic domain (1025aa-End)

Promoter

T7

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

Plasmid

#69906

Purpose

Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ciRS7 (CDR1-AS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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vYM112_PB-pEF1a-optiPIN2-HA(Hygro)

Plasmid

#160045

Purpose

For expressing the active auxin pump PIN2

Depositor

Michael Elowitz

Article

Ma et al Cell. 2022 Mar 17;185(6):967-979.e12. do

Insert

PIN2

Use

Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

codon optimized

Promoter

pEF1s

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAdEasy Flag GCN5

Plasmid

#14106

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

E189G and K195N

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D -IgK-TurboID-HA

Plasmid

#219810

Purpose

Secreted HA-tagged TurboID for extracellular proximity labeling

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

IgK (Igk Mouse)

Use

AAV

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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Chx10BP-LCL-EGFP

Plasmid

#73992

Purpose

Cre-dependent reporter plasmid for bipolar cells in the retina

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Chx10BP-loxP-mCherry-loxP-EGFP

Use

Cre/Lox

Expression

Mammalian

Promoter

Chx10BP (chx10 enhancer)

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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ptagRFP-MS2coatProtein

Plasmid

#103831

Purpose

Live cell reader for MS2 loop RNA production

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

MS2 coat protein (MCP) (cp )

Tags

tagRFP

Expression

Mammalian

Mutation

MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

Promoter

CMV

Available Since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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