We narrowed to 32,675 results for: eng

1. pBTK503

   Plasmid

   #110616

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pTSin EGFP

   Plasmid

   #127695

   Purpose

   Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Available Since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pBTK619

   Plasmid

   #110619

   Purpose

   BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter

   Depositor

   Jeffrey Barrick

   Article

   Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 10, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSin Helper

   Plasmid

   #127694

   Purpose

   Plasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Glycoproteins
   

   Use

   Synthetic Biology

   Available Since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pSin Capsid

   Plasmid

   #127693

   Purpose

   Plasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Capsid
   

   Use

   Synthetic Biology

   Available Since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pSRE SSG

   Plasmid

   #127692

   Purpose

   Plasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.

   Depositor

   Justin English, Bryan Roth

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   Sindbis Structural Genome
   

   Expression

   Mammalian

   Available Since

   July 3, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV-TRE-DIO-FLPo

   Plasmid

   #118027

   Purpose

   AAV vector for sparse and bright labeling in a cell-type specific manner

   Depositor

   Minmin Luo

   Article

   Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

   Insert

   TRE-DIO-FLPo
   

   Use

   AAV

   Promoter

   TRE

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJY-RpABE

   Plasmid

   #112872

   Purpose

   binary vector for ABE expression in Arabidopsis

   Depositor

   Jin-Soo Kim, Jae-Young Yun

   Article

   Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

   Inserts

   RPS5A promoter
   ABE7.10
   U6-sgRNA cassette
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #176273

   Purpose

   Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (sgTet) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-3XpolyA-3XPC-miniCMV-mCherry-WPRE-insulator

    Plasmid

    #168291

    Purpose

    optimized miniCMV (OminiCMV)

    Depositor

    Hui Yang

    Article

    Gao et al Nat Cell Biol. 2021 Jan;23(1):99-108. do

    Insert

    mCherry
    

    Expression

    Mammalian

    Promoter

    miniCMV

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-mCherry-NLS

    Plasmid

    #165442

    Purpose

    Expresses nuclear mCherry from CMV promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

    Insert

    mCherry-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. BPK764

    Plasmid

    #65767

    Purpose

    Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Promoter

    T7 (x2)

    Available Since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-R2Tg_opt-3xFlag

    Plasmid

    #223251

    Purpose

    Mammalian expression of C-terminally Flag-tagged optimized R2Tg protein

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg protein_opt
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

    Plasmid

    #176268

    Purpose

    Expression of dCas12f-VPR-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    dCas12f
    

    Use

    Lentiviral

    Tags

    HA-VPR-mCherry

    Expression

    Mammalian

    Mutation

    D326A, D510A

    Promoter

    PGK

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAcGHLT-B-DDB1

    Plasmid

    #48638

    Purpose

    Expresses Full Lenght DDB1 in Insect Cells (ampR)

    Depositor

    Ning Zheng

    Article

    Li et al Cell. 2006 Jan 13;124(1):105-17.

    Insert

    DDB1 (DDB1 Human)
    

    Expression

    Insect

    Available Since

    Oct. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pEF1a-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223252

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Pet22b-mEGFP

    Plasmid

    #166439

    Purpose

    Bacterial expression of 6x His tagged mEGFP

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP
    

    Expression

    Bacterial

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL2- Empty control -SV40-Luc

    Plasmid

    #26280

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Type

    Empty backbone

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDIRECT_23A

    Plasmid

    #91138

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMV-LEU2

    Plasmid

    #65334

    Purpose

    standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker LEU2 which could be released and ligated to series of TUs

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    LEU2
    

    Use

    Synthetic Biology

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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