We narrowed to 6,919 results for: dna plasmids

1. Cdk12_intron3_sg2_pX330

   Plasmid

   #127174

   Purpose

   sgRNA that cuts within intron 3 of mouse Cdk12 genomic locus in pX330 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cdk12_intron4_sg1_pX458

   Plasmid

   #127175

   Purpose

   sgRNA that cuts within intron 4 of mouse CDK12 genomic locus in pX458 backbone

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cdk12 Intron 4 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Promoter

   U6 Promoter

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCIneo-hCHD7

   Plasmid

   #89458

   Purpose

   constitutive expression of human CHD7 in mammalian cells

   Depositor

   Peter Scacheri

   Insert

   CHD7 (CHD7 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. MLH1

   Plasmid

   #32859

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   MLH1 (MLH1 Human)
   

   Tags

   His (with TEV cleavage)

   Expression

   Bacterial

   Mutation

   contains amino acids 486-751

   Available Since

   Feb. 22, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. TTN/pAS2-1

   Plasmid

   #98127

   Purpose

   titin in DNA-binding domain (BD) vector, used for yeast-two hybrid

   Depositor

   Rita Shiang

   Insert

   TTN (TTN Human)
   

   Expression

   Yeast

   Available Since

   Aug. 21, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pLgw ELK1Y158A-V5-EcoDam

   Plasmid

   #98609

   Purpose

   Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Insert

   ELK1Y158A (Elk1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   V5 and Dam (DNA adenine methyltransferase)

   Expression

   Mammalian

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLgw EcoDam-V5-ELK1Y158A

   Plasmid

   #98608

   Purpose

   Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Insert

   ELK1Y158A (Elk1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCambia-dLB-kanR-GFP

   Plasmid

   #223177

   Purpose

   Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

   Depositor

   Andreas Stuermer

   Type

   Empty backbone

   Use

   Ti plasmid for agrobacterium

   Expression

   Plant

   Available Since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pLexA::nud-1

   Plasmid

   #11339

   Depositor

   Guy Caldwell

   Insert

   nud-1 (nud-1 Nematode)
   

   Tags

   LexA DNA Binding Domain

   Expression

   Yeast

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pLexA::lis-1

    Plasmid

    #11338

    Depositor

    Guy Caldwell

    Insert

    lis-1 (lis-1 Nematode)
    

    Tags

    LexA DNA Binding Domain

    Expression

    Yeast

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-short_BrokenHeart (no BC)

    Plasmid

    #86950

    Purpose

    AAV transfer plasmid containing the BrokenHeart construct: a hyperpiggybac donor transposon interrupting the tdTomato fluorophore. Transposase activity rescues coding sequence.

    Depositor

    Rob Mitra, Joseph Dougherty

    Article

    Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    Brokenheart construct
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BE3-hA3A-R128A

    Plasmid

    #132944

    Purpose

    Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    BE3-hA3A(R128A)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    BE3-hA3A(R128A)

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BE3-hA3A-Y130F

    Plasmid

    #132945

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    BE3-hA3A(Y130F)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    BE3-hA3A(Y130F)

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-noSV40-humanEC1.45_min1-2_4XEboxMutant

    Plasmid

    #215026

    Purpose

    Firefly luciferase enhancer reporter plasmid with minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with E-boxes mutated
    

    Use

    Luciferase

    Mutation

    4X E-box mutations within Coordinator motifs

    Promoter

    SV40

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAID1.2-EF1a-NmClover3-hsLem2

    Plasmid

    #179524

    Purpose

    Express OsTIR1 and mClover3-mAID-hsLem2 in mammalian cells under the control of EF1a promoter

    Depositor

    Yasushi Hiraoka

    Article

    Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

    Insert

    LEMD2 (LEMD2 Human)
    

    Tags

    mClover3-mAID

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BE3-YE1

    Plasmid

    #132943

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    BE3-(W90Y-R126E)
    

    Use

    CRISPR; Base editor

    Expression

    Mammalian

    Mutation

    BE3-(W90Y-R126E)

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JARID1BA

    Plasmid

    #74646

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1BA (KDM5B Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    Leu101-(Gly)4-Ala374 deletion mutant

    Promoter

    PH

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. JARID1BA

    Plasmid

    #74648

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JARID1BA (KDM5B Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    Leu101-(Gly)4-Ala374 deletion mutant

    Promoter

    PH

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only