We narrowed to 14,310 results for: eng
-
Plasmid#67305PurposeBlue light dependent photoswitching systemDepositorAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only
-
MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5
Plasmid#135455PurposeBacterial expression of uTEV1Δ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
TagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability, S153N mutation…Promotertac promoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
lentiCRISPR v2-sgSLC7A11/xCT-2
Plasmid#161819Purposeknock out SLC7A11/xCT in mammalian cellsDepositorInsertsolute carrier family 7 member 11 (SLC7A11 Human)
UseCRISPR and LentiviralAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY003 (pFnCpf1_delta Cas)
Plasmid#69974PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes
UseCRISPRExpressionBacterialMutationCas genes are deleted from locusAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330A_D10A-1x4
Plasmid#58774PurposeExpresses Cas9 nickase and gRNADepositorInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTags3xFLAGExpressionMammalianMutationD10A nickase-converting mutationPromoterCBhAvailable sinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRSET FLII12Pglu-600u
Plasmid#13563DepositorInsertFLIPglu F16A Y12S-ECFP-D13R
TagsCitrineExpressionBacterialMutationF16A ECFP insertion in binding proteinAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCEP4-BaLgp160
Plasmid#100919PurposeMammalian expression plasmid for Env from the BaL HIV-1 isolateDepositorInsertHIV-1 (BaL) Env
TagsCD5 leader sequenceExpressionMammalianMutationCodon-optimized synthetic genePromoterCMVAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9
Plasmid#175523PurposeCo-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertLAMP1-HaloTag11 and Tomm20-HaloTag9
ExpressionMammalianMutationHaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA GASTM-3-RVG-10-Lamp2b-HA
Plasmid#71295PurposeEncodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.DepositorInsertLamp2b (LAMP2 Human)
TagsGASTM mutated glycosylation motif, HA, Lamp2 sig…ExpressionMammalianPromoterCMVAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v6-10 (fl)
Plasmid#137820Purposeexpression of CD44 proteins in mammalian cellsDepositorAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hM₃O-IP
Plasmid#46645DepositorInserthM₃O (POU5F1 Human)
UseRetroviralTagsIRES Puro and human MyoD (aa 1-62)ExpressionMammalianMutationcontains a fragment of human MyoD (aa1-62) fused …Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
1352wUV2 F1bbs kan GCGTGGNNN
Plasmid#18753DepositorInsert3 tandem zinc fingers
ExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…Available sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
1352wUV2 F3bbs kan NNNTGGGCGk
Plasmid#18756DepositorInsert3 tandem zinc fingers
ExpressionBacterialMutationA Kan cassette (1090bp) is inserted between two b…Available sinceJuly 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
MG1655 ΔendA ΔrecA
Bacterial strain#37853DepositorBacterial resistanceNoneAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
eScaf
Bacterial strain#220921PurposecssDNA production strainDepositorBacterial resistanceNoneSpeciesEscherichia coliAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKT07_AAV-hU6-AsDR-crTSG1-EFS-Cre-T2A-hNGFR
Plasmid#256630PurposeAAV vector for delivery of AsCas12a crTSG1 guide array and expressing Cre using EFS promoterDepositorInsertcrTrp53, crPten, crApc, crRb1
UseAAVPromoterhumanU6, EFS-NSAvailable sinceJune 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
pKT07_AAV-hU6-AsDR-EFS-Cre-T2A-hNGFR
Plasmid#256629PurposeAAV vector for delivery of AsCas12a guides and expressing Cre and hNGFR using EFS promoterDepositorTypeEmpty backboneUseAAVAvailable sinceJune 9, 2026AvailabilityAcademic Institutions and Nonprofits only -
C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)
Plasmid#242662PurposeCBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4DepositorInsertpAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
UseAAV and CRISPRTagsBPNLSMutationVRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…PromoterCBhAvailable sinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only