We narrowed to 32,675 results for: eng

1. IVT-donor_opt(R2Tg)-GFP

   Plasmid

   #223246

   Purpose

   In vitro transcription of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Use

   In vitro transcription

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #176272

   Purpose

   Expression of non-targeting sgRNA of CasMINI

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI non-targeting sgRNA (sgLacZ) and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. OA984-HA

   Plasmid

   #120362

   Purpose

   Expresses a his-tagged single chain antibody 1C19 that targets all four DENV serotypes and expresses red fluorescent protein reporter

   Depositor

   Omar Akbari

   Article

   Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.

   Insert

   1C19 his-tagged single chain antibody
   

   Use

   Unspecified

   Promoter

   AAEL010782 carboxypeptidase A (CPA) gene Promoter

   Available Since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZapLck biosensor

   Plasmid

   #131584

   Purpose

   Lck FRET Biosensor. To visualize Lck kinase activity in mammalian cells by FRET

   Depositor

   Yingxiao Wang

   Article

   Wan et al Sci Adv. 2019 Jun 19;5(6):eaau2001. doi:

   Insert

   ZapLck biosensor
   

   Use

   Lentiviral

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-pCAG-donor(R2Tg)-GFP(intron)

   Plasmid

   #223248

   Purpose

   Mammalian expression of the R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSP1673

   Plasmid

   #65769

   Purpose

   Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Bacterial

   Promoter

   T7 (x2)

   Available Since

   June 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDIRECT_25F

   Plasmid

   #91143

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-R2Tg-3xFlag

   Plasmid

   #223250

   Purpose

   Mammalian expression of C-terminally Flag-tagged R2Tg protein

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg protein
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-neo

   Plasmid

   #166467

   Purpose

   Empty vector to express overexpression cDNA

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nanog-2A-mCherry

    Plasmid

    #48680

    Purpose

    For knock-in donor vector by CRISPR/CAS system

    Depositor

    Rudolf Jaenisch

    Article

    Yang et al Cell. 2013 Aug 27. pii: S0092-8674(13)01

    Insert

    Nanog (Nanog Mouse)
    

    Use

    CRISPR

    Tags

    Cherry

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-INPP5E

    Plasmid

    #171948

    Purpose

    Expresses INPP5E in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    INPP5E (INPP5E Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBTK620

    Plasmid

    #110620

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Jan. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBTK621

    Plasmid

    #110621

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Sept. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pDIRECT_21A

    Plasmid

    #91128

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBTK519

    Plasmid

    #110603

    Purpose

    BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pBTK107

    Plasmid

    #110575

    Purpose

    BTK Type 2 promoter for golden gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    CP25 Promoter with RBS
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pET28a-MBP-CBX1-eReader

    Plasmid

    #135089

    Purpose

    Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered
    

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBTK301

    Plasmid

    #110593

    Purpose

    BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Bba_B0015 Terminator
    

    Use

    Synthetic Biology

    Available Since

    Feb. 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMBA334

    Plasmid

    #214746

    Purpose

    High copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

    Depositor

    Tae Seok Moon

    Article

    Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

    Insert

    BBa_J23119-RiboJ-RBS(21992)-gfpmut3
    

    Expression

    Bacterial

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-40XUAS-Luc

    Plasmid

    #166455

    Purpose

    Luciferase reporter

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    40XUAS , Luciferase
    

    Use

    Lentiviral

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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