We narrowed to 11,399 results for: nar;

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pEM9_CL7_Bac_2NLS-iCas12a (LbCas12a)-4NLS

Plasmid

#244844

Purpose

Bacterial expression of iCas12a LbCas12a with 2xSV40 nuclear localization sequence for protein purification

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

iCas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

F863V/D952N/C965Y/V1011A/Q1108L/A1113V/S1132T/S12…

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pEM5_CL7_Bac_2NLS-Lbm6 (LbCas12a)-4NLS

Plasmid

#244840

Purpose

Bacterial expression of Lbm6 LbCas12a with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Lbm6-Cas12a

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 4xSV40 NLS

Expression

Bacterial

Mutation

D535G/S551F/D665N

Promoter

T7

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b PhiC31 attP41

Plasmid

#242881

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 4a PhiC31 attP41

Plasmid

#242882

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA AAV 3b PhiC31 attP41

Plasmid

#242883

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human AAVS1 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4b Bxb1 attB38

Plasmid

#242879

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a Bxb1 attB38

Plasmid

#242878

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal Bxb1 attB site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for Bxb1 attB site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pU6 pegRNA ROSA 4a PhiC31 attP41

Plasmid

#242880

Purpose

Mammalian expression of epegRNA for twin prime editing of half the minimal PhiC31 attP site targeting the human ROSA26 site

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

epegRNA for PhiC31 attP site

Expression

Mammalian

Mutation

none

Promoter

U6

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHS641_Flex-Cas12a-ABE

Plasmid

#244846

Purpose

Mammalian expression of Flex-Cas12a adenine base editor

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Flex-dCas12a-ABE

Use

CRISPR

Tags

SV40 NLS-ABE8e

Expression

Mammalian

Mutation

G146R/R182V/D535G/S551F/D665N/E795Q/D832A

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEM6_CL7_Bac_2NLS-RVQ (LbCas12a)-2NLS

Plasmid

#244841

Purpose

Bacterial expression of LbCas12a-RVQ with 2xSV40 NLS at N-terminus and 4xSV40 at C-terminus

Depositor

Jennifer Doudna

Article

Ma et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

LbCas12a-RVQ

Use

CRISPR

Tags

10xHis-CL7-2xSV40 NLS and 2xSV40 NLS

Expression

Bacterial

Mutation

G146R/R182V/E795Q

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SpRY-IP

Plasmid

#235994

Purpose

Expresses Cas9-SpRY in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

SpRY

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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iSM-IP

Plasmid

#235990

Purpose

Expresses iSM (iSpyMac) in mammalian cells

Depositor

Tobias Cantz

Article

Eggenschwiler et al Nucleic Acids Res. 2025 Aug 11;53(15):gk

Insert

iSM

Use

CRISPR

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-PCNAL2 (pc3385)

Plasmid

#213140

Purpose

Expresses human PCNA miRFP670 tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Rausch et al Nucleic Acids Res. 2021 Dec 16;49(22):12

Insert

PCNA (PCNA Human)

Tags

miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pMDC32B-B/c

Plasmid

#227963

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Expression

Plant

Promoter

35S

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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Const.6 EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc-PS9

Plasmid

#231910

Purpose

For packaging EGFP, dTomato, TagBFP and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-T2A-TagBFP-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

Plasmid

#231909

Purpose

For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP-P2A-dTomato-IRES-Luc

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-RNAgateDel-sgEIF3D

Plasmid

#222137

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

V291-F306 deletion

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-S528D-S529D-sgEIF3D

Plasmid

#222127

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S528D, S529D

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-CtermDel-sgEIF3D

Plasmid

#222131

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

161 + 527 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-NtermDel-sgEIF3D

Plasmid

#222123

Purpose

EIF3D variant overexpression vector with sgRNA targeting eIF3D

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Mutation

S161 truncation

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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