We narrowed to 27,436 results for: MUT
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Plasmid#114184PurposeORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.DepositorInsertORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
TagsYFPExpressionMammalianMutationT184M/L273D/L276DAvailable SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_VPEVS
Plasmid#105863PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
ExpressionMammalianMutationmutated heavy chain of mouse IgG3 with five mutat…PromoterhEF1-HTLV promAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_ILGGP
Plasmid#105855PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
ExpressionMammalianMutationmutated heavy chain of mouse IgG1 with five mutat…PromoterhEF1-HTLV promAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125/147L-siResist
Plasmid#49856PurposeEncodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
ExpressionMammalianMutationMethionine100, Methionine 125, and Methionine 147…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125L-siResist
Plasmid#49855PurposeEncodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
ExpressionMammalianMutationMethionine100 and Methionine 125 mutated to Leuci…PromoterCMVAvailable SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-SF3B1-K700E-reporter
Plasmid#200318PurposeA fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.DepositorInsertBSD-2A-EGFPi
UseLentiviralExpressionMammalianPromoterEF1-aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPFO*
Plasmid#120400PurposeExpresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.DepositorInsertMutant form of Perfringolysin O
TagsHis6 tagExpressionBacterialMutationAmino acids 1-27 are deleted, starts at amino aci…Available SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB P305L
Plasmid#88887PurposeSCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralMutationProline to Leucine mutation @ 305th amino acidPromoterMSCV promoterAvailable SinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-3xFLAG-NFATC2IP D394R
Plasmid#221211PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interactionDepositorInsertNFATC2IP (NFATC2IP Human)
Tags3XFlagExpressionMammalianMutationD394R (charge-reverting mutation for interaction …PromoterCMVAvailable SinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-eGFP-NFATC2IP D394R
Plasmid#221436PurposeMammalian expression vector of eGFP tagged NFATC2IP ORF with D394R point mutation. Charge-reverting mutation for UBC9 R13D mutant for interactionDepositorInsertNFATC2IP (NFATC2IP Human)
TagseGFPExpressionMammalianMutationD394R (charge-reverting mutation for interaction …PromoterCMVAvailable SinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMtb MCM
Plasmid#138296PurposeExpression of Mtb MCM small and large subunitDepositorInsertsmutA
mutB
TagsHis tag and TEV cleavage siteExpressionBacterialMutationinserted TEV cleavage site between His-tag and m…PromoterT7Available SinceApril 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB6_EZH2
Plasmid#84014PurposeEZH2 minigene to test splicing eventDepositorAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL452(hygro)-Sf3b1-K700K
Plasmid#90426Purposeselectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)DepositorInsertSf3b1 - left and right homology arms (Sf3b1 Mouse)
UseHomology-directed repair vectorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L-MM
Plasmid#89611PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416DepositorAvailable SinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX
Plasmid#112762Purposeexpress GFP-4EBP1-Rheb15(CAAX mutation)DepositorInsertFlag-GFP-4EBP1-Rheb15 (CAAX mutant) (EIF4EBP1 Human)
UseLentiviralTagsRheb15(CAAX mutant)MutationCAAX mutation to prevent lipid localizationAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-M100/125/147/213-siResist
Plasmid#49858PurposeEncodes human connexin 43 with M100, M125, M147, and M213 mutated to L and wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
ExpressionMammalianMutationMethionine100, Methionine 125, Methionine 147, an…PromoterCMVAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE5max-SF3B1-K700E
Plasmid#200317PurposeExpresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.DepositorInsertsK700E-ng1+pg2
PEmax-2A-MLH1dn
ExpressionMammalianPromoterCMVAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
c1GFP-W53XKir7.1
Plasmid#107183PurposeGenerates Nonsense mutant cloneDepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C_R169W-mCherry-FRB
Plasmid#185984PurposeMammalian expression of human KIF1C with R169W mutation and mCherry and FRB tags.DepositorInsertKIF1C (KIF1C Human)
TagsmCherry and FRBExpressionMammalianMutationR169W - mutation causing HSP (SPG58) and silent m…Available SinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
EXT1- D164A-pLJC2-3XFLAG
Plasmid#215828PurposeEctopic expression of catalytically inactive mutant (EXT1-D164A-FLAG) in mammalian cellsDepositorInsertExostosin glycosyltransferase 1 Mutant (EXT1 Human)
UseLentiviralTags3X FlagExpressionMammalianAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only