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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 21,308 results for: cdna

Showing: 2741 - 2760 of 21308 results

  1. pcDNA3-Lyso-AktAR2

    Plasmid
    #64933
    Purpose
    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.
    Depositor
    Jin Zhang
    Article
    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1
    Insert
    LAMP1-AktAR2 (LAMP1 Human, Synthetic, Budding Yeast)
    Use
    Tags
    Cerulean3, Full-length LAMP1, and cpVenus[E172]
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64933-plasmid-map-sequence-id-109712

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  5. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid
    #92305
    Purpose
    Expression of humanized Mb3Cpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huMb3Cpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92305-plasmid-map-sequence-id-181650

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  7. pcDNA3-LYRa11-RBD-8h

    Plasmid
    #161822
    Purpose
    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Receptor-binding domain (RBD) of spike protein S
    Use
    Tags
    GSG linker and 8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Human codon optimized RBD (a.a. T324-D522)
    Promoter
    CMV
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    161822-plasmid-map-sequence-id-316758

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  8. pcDNA3.1-MS2-HA-HsMex3B_AD

    Plasmid
    #148478
    Purpose
    Mammalian Expression of HsMex3B
    Depositor
    Elisa Izaurralde
    Insert
    HsMex3B (MEX3B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148478-plasmid-map-sequence-id-409989

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  14. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid
    #92303
    Purpose
    Expression of humanized TsCpf1 and crRNA
    Depositor
    Feng Zhang
    Insert
    huTsCpf1
    Use
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92303-plasmid-map-sequence-id-181636

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  15. pcDNA3.1(-)-mRho.V5.mER.hOr47a

    Plasmid
    #126472
    Purpose
    Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells
    Depositor
    Bill Hansson
    Article
    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.
    Insert
    Or47a (Or47a Fly)
    Use
    Tags
    H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…
    Expression
    Mammalian
    Mutation
    codon optimization for H. sapiens
    Promoter
    CMV
    Available since
    June 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    126472-plasmid-map-sequence-id-246570

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  17. pcDNA3.1(+)Gαi2-91-NLuc

    Plasmid
    #207149
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2
    Depositor
    Tom Van Loy, Dominique Schols
    Article
    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76
    Insert
    GNAI2 (GNAI2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position L(91)Q
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    207149-plasmid-map-sequence-id-410273

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Showing: 2741 - 2760 of 21308 results