We narrowed to 150,487 results for: ins

1. pMSCV-FKBP-SYNDAPIN2

   Plasmid

   #221311

   Purpose

   Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   SYNDAPIN2 (pLxIS) (Pacsin2 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG-4xFKBP

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOCC305

   Plasmid

   #118898

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with 3C and C-terminal HIS6, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-TwinStrep-MBP-3C-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and TwinStrep-MB…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. RIP Cre-ER (DM#265)

   Plasmid

   #15029

   Depositor

   Douglas Melton

   Article

   Dor et al Nature. 2004 May 6. 429(6987):41-6.

   Insert

   Rat insulin promoter (RIP) (Ins2 Rat)
   

   Use

   Cre/Lox; For making transgenic constructs

   Tags

   Cre-ER (TM)

   Expression

   Mammalian

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-mCherry::LANS4

   Plasmid

   #60785

   Purpose

   Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

   Insert

   LANS4
   

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFX-mCherry-KRasCT

   Plasmid

   #175835

   Purpose

   Express mCherry with plasma membrane-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   mCherry with plasma membrane-targeting sequence
   

   Tags

   C-terminal hypervariable region of K-Ras 4B

   Expression

   Mammalian

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW6.01

   Plasmid

   #62882

   Purpose

   Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   GFP::MEG-3
   

   Tags

   GFP

   Expression

   Worm

   Promoter

   meg-3

   Available Since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-hKCNE1

   Plasmid

   #53050

   Purpose

   Expresses beta subunit for IKs channel in mammalian cells

   Depositor

   Michael Sanguinetti

   Article

   Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

   Insert

   Potassium voltage-gated channel subfamily E member 1 (KCNE1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC57-Amp_CRISPIE donor B2

   Plasmid

   #172843

   Purpose

   CRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mRuby3
   

   Use

   CRISPR

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

   Plasmid

   #172068

   Purpose

   Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   GFP11x7
   

   Expression

   Insect

   Available Since

   Oct. 20, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFX-mKate2-Rab11

    Plasmid

    #174463

    Purpose

    Express ｍKate2 with recycling endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mKate2 with recycling endosome-targeting sequence
    

    Tags

    small GTPase (Rab11)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. DT240

    Plasmid

    #80414

    Purpose

    Expresses 240 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFX-mito-eqFP650

    Plasmid

    #174179

    Purpose

    Express eqFP650 with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    eqFP650 with mitochondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T-2/hGGA3(GAT)

    Plasmid

    #79436

    Purpose

    Expresses GST-tagged GAT domain of GGA3 (107-286 aa) in E.coli

    Depositor

    Kazuhisa Nakayama

    Article

    Takatsu et al Biochem J. 2002 Jul 15;365(Pt 2):369-78.

    Insert

    GGA3 (GGA3 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 107-286

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFX-mCherry-Rab11

    Plasmid

    #174461

    Purpose

    Express mCherry with recycling endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with recycling endosome-targeting sequence
    

    Tags

    small GTPase (Rab11)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFX-mCherry-Rab7

    Plasmid

    #174458

    Purpose

    Express mCherry with late endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with late endosome-targeting sequence
    

    Tags

    small GTPase (Rab7)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFX-EGFP-Sec61β

    Plasmid

    #174466

    Purpose

    Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    Sirius with endoplasmic reticulum-targeting sequence
    

    Tags

    Sec61 subunit beta

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL1 Aquamarine

    Plasmid

    #106285

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Aquamarine
    

    Tags

    6xHis and TEV cleavage (N terminal on insert)

    Expression

    Bacterial

    Mutation

    T65S and H148G

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET17b-yebST

    Plasmid

    #175804

    Purpose

    Complementation of yebST knockout in E. coli K-12

    Depositor

    Damian Ekiert, Ian Henderson, Gira Bhabha

    Article

    Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

    Insert

    yebST
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    silent mutations to introduce restriction sites

    Promoter

    T7

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAB076i5

    Plasmid

    #79199

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2+3); Ppro1

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFX-mito-roGFP

    Plasmid

    #174181

    Purpose

    Express roGFP with mitochondria-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    roGFP with mitchondria-targeting sequence
    

    Tags

    the mitochondria-targeting sequence of the cytoch…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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