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pET28a-SznF H407A H409A H448A

Plasmid

#139566

Purpose

expressed SznF H407A H409A H448A in E. coli

Depositor

Emily Balskus

Article

Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

Insert

SznF H407A H409A H448A

Tags

hexahistidine

Expression

Bacterial

Mutation

changed glutamate 215 to alanine

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFast-dCry-V5His

Plasmid

#136240

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Insect

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMX234

Plasmid

#23006

Depositor

Linda Wordeman

Article

Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

Insert

mRFP-CenpB

Tags

mRFP1.0

Expression

Mammalian

Mutation

deleted CenpB amino acids 169-606, T145S

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pITRSF4

Plasmid

#89699

Purpose

For production of GFP with "GLP" N-tag and "TRNGL" for C-tag for cyclisation control.

Depositor

Hideo Iwai

Article

Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

Insert

SMT3_GLP_H6_GFP_TRNGL

Tags

SMT3

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJTRSF50

Plasmid

#89700

Purpose

For production of GFP with "GLP" N-tag and "TRNCL" for C-tag for cyclisation control.

Depositor

Hideo Iwai

Article

Mikula et al FEBS Lett. 2017 Mar 30. doi: 10.1002/187

Insert

SMT3_GLP_H6_GFP_TRNCL

Tags

SMT3

Expression

Bacterial

Promoter

T7

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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recoverin1a

Plasmid

#83811

Purpose

express zebrafish recoverin1a

Depositor

Stephan Neuhauss

Article

Zang et al Open Biol. 2015 Aug;5(8). pii: 150086. d

Insert

recoverin1a (rcvrna Zebrafish)

Expression

Bacterial

Mutation

*(see below)

Available Since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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recoverin2a

Plasmid

#83813

Purpose

express zebrafish recoverin2a

Depositor

Stephan Neuhauss

Article

Zang et al Open Biol. 2015 Aug;5(8). pii: 150086. d

Insert

recoverin2a (rcvrn2 Zebrafish)

Expression

Bacterial

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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SK-YFP-NM-A

Plasmid

#48664

Purpose

Bacterial NM repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

NM prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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SK-YFP-TD-A

Plasmid

#48666

Purpose

Bacterial TD repression YFP reporter: protospacer A

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TD prototspacer A/PAM with reporter EYFP

Use

CRISPR

Promoter

lambda pR promoter

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pSKB3.TM1468

Plasmid

#11431

Depositor

Sung-Hou Kim

Article

Schulze-Gahmen et al Proteins. 2003 Mar 1. 50(4):526-30.

Insert

fatty acid binding protein

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pMOD3-proD/tdTomato-nat

Plasmid

#120326

Purpose

Bacterial tdTomato labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

tdTomato

Expression

Bacterial

Promoter

proD

Availability

Academic Institutions and Nonprofits only

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pMOD3-mCardinal-Sh_ble

Plasmid

#120334

Purpose

Bacterial mCardinal promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mCardinal

Expression

Bacterial

Promoter

(None)

Availability

Academic Institutions and Nonprofits only

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pMOD3-mNeptune2-nat

Plasmid

#120335

Purpose

Bacterial mNeptune2 promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Expression

Bacterial

Promoter

(None)

Availability

Academic Institutions and Nonprofits only

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pMOD3-mNeptune2-Sh_ble

Plasmid

#120336

Purpose

Bacterial mNeptune2 promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Expression

Bacterial

Promoter

(None)

Availability

Academic Institutions and Nonprofits only

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pET29a-human histone 4-in-One

Plasmid

#223237

Purpose

Polycistronic bacterial coexpression construct for human His-Sumo-H2A, H2B, H3, and Dual-StrepII-H4

Depositor

Weixing Zhao

Inserts

His6-VSVG-Histone H2A
Histone H2B
Histone H3
Histone H4-TEV-His6-Dual StrepII

Tags

Dual StrepII, His6, TEV protease site, and VSVG

Expression

Bacterial

Mutation

A103G

Promoter

T7

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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mTFP1-pBAD

Plasmid

#54553

Purpose

Localization: Protein Expression Vector , Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

Type

Empty backbone

Tags

6xHis and mTFP1

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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CMV-Tol2 transposase

Plasmid

#158774

Purpose

Expression of Tol2 transposase

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

Tol2 tranposase

Expression

Mammalian

Mutation

Tol2 codon usage frequency well suitable for muri…

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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mCerulean3-C1

Plasmid

#54605

Purpose

Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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CAG::PhiC31

Plasmid

#140506

Purpose

Encodes PhiC31 recombinase driven by the CAG promoter

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

CAG promoter

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pSL3287

Plasmid

#139750

Purpose

Base Cas12a CRISPRi plasmid

Depositor

Himadri Pakrasi

Article

Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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