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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,709 results for: actin

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Showing: 2761 - 2780 of 9709 results

  5. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid
    #131137
    Purpose
    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.
    Depositor
    Mark Peifer
    Publication
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    Crk (Crk Fly)
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131137-plasmid-map-sequence-id-258861

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  6. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid
    #172473
    Purpose
    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.
    Depositor
    Sean R Collins
    Publication
    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    Use
    Piggybac transposon
    Tags
    polybasic and CAAX motif from Kras, tdKatushka2, …
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Sept. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172473-plasmid-map-sequence-id-342817

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  7. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid
    #72299
    Purpose
    Express gene of interest under CB promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer
    Available since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    72299-plasmid-map-sequence-id-131901

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  8. iLOV-Lifeact-7

    Plasmid
    #54746
    Purpose
    Localization: Actin, Excitation: 450, Emission: 495
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lifeact-iLOV
    Expression
    Mammalian
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. tdEos-Lifeact-7

    Plasmid
    #54527
    Purpose
    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lifeact-tdEos
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  10. pCMVtrunc msfGFPΔC10-Utr28-222

    Plasmid
    #231558
    Purpose
    Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Publication
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Utrophin calponin homology domain (UTRN Human)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231558-plasmid-map-sequence-id-459698

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  11. pCMVtrunc LifeactΔC4-msfGFPΔN7

    Plasmid
    #231555
    Purpose
    Mammalian expression of C-terminally truncated Lifeact fused to N-terminally truncated monomeric superfolder GFP for actin filament organization measurements using fluorescence polarization microscopy
    Depositor
    Manos Mavrakis
    Publication
    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.
    Insert
    Lifeact (ABP140 Budding Yeast)
    Tags
    msfGFP
    Expression
    Mammalian
    Promoter
    CMVtrunc
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231555-plasmid-map-sequence-id-459696

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  12. pCDH-CB-IRES-copGFP-T2A-Puro

    Plasmid
    #72299
    Purpose
    Express gene of interest under CB promoter
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    chicken beta-actin + CMV enhancer
    Available since
    Jan. 25, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    72299-plasmid-map-sequence-id-131901

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  13. mEmerald-Espin-like-N-18

    Plasmid
    #54087
    Purpose
    Localization: Actin Bundling, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Espin-like
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. mCitrine-VASP-5

    Plasmid
    #56571
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 516, Emission: 529
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VASP
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    56571-plasmid-map-sequence-id-316715

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  16. mTagRFP-T2-VASP-5

    Plasmid
    #58045
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    VASP
    Tags
    mTagRFP
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. mCerulean-VASP-5

    Plasmid
    #55393
    Purpose
    Localization: Actin/Focal Adhesions, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VASP
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    A209T in VASP
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Academic Institutions and Nonprofits only
    55393-plasmid-map-sequence-id-368570

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  18. mCherry-MCPB2-C-18

    Plasmid
    #55083
    Purpose
    Localization: Actin Capping Protein , Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    MCPB2
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. mCherry-Espin-Like-C-18

    Plasmid
    #62744
    Purpose
    Localization: Actin Bundling , C1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    EspinLike
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pEGFP-PH-SWAP70(R223E, R224E)

    Plasmid
    #84583
    Purpose
    Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding
    Depositor
    Geert van den Bogaart
    Publication
    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Pleckstrin-homology domain, residues 209-306, mut…
    Promoter
    CMV
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 2761 - 2780 of 9709 results