We narrowed to 3,225 results for: bad

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pDD143

Plasmid

#119880

Purpose

Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

Insert

pBAD_dCas9sth1

Expression

Bacterial

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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Sp_PE

Plasmid

#212038

Purpose

Bacterial expression of the PE2 proteins for genome editing in E. coli

Depositor

Quanjiang Ji

Article

Zhang et al Nat Commun. 2024 Jan 27;15(1):825. doi:

Insert

PE2

Use

CRISPR

Promoter

L-arabinose-inducible promoter PBAD

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFREE_Amp

Plasmid

#92051

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pDae029 (eMutaT7[TadA-8e])

Plasmid

#187620

Purpose

Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

TadA-8e-T7RNAP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFREE_Cm

Plasmid

#92052

Purpose

Allows inducible expression of gRNAs and Cas9 for plasmid curing.

Depositor

Morten Norholm

Article

Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

Inserts

Cas9
gRNA array

Use

CRISPR

Promoter

pRhamBAD and ptet

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSU-araC-Cas9

Plasmid

#120423

Purpose

Multigene editing in the Escherichia coli genome using the CRISPR-Cas9 system

Depositor

Sheng Yang

Article

Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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gamma Synuclein-VC

Plasmid

#89734

Purpose

expresses human gamma Synuclein fused to the C-terminal part of Venus

Depositor

Tiago Outeiro

Article

Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

Insert

SNCG (SNCG Human)

Tags

C-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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VN-gamma Synuclein

Plasmid

#89733

Purpose

expresses human gamma Synuclein fused to the N-terminal part of Venus

Depositor

Tiago Outeiro

Article

Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

Insert

SNCG (SNCG Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pJS96

Plasmid

#207588

Purpose

GFP-TonBH20A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonBH20A

Expression

Bacterial

Mutation

H20A

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-Para

Plasmid

#218363

Purpose

Empty backbone for Tn7-site insertion with araC-ParaBAD expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLsCas13a-gRNA-NT

Plasmid

#164857

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-NT

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJS119

Plasmid

#207592

Purpose

GFP-TonB deltaII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonB deltaII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJS104

Plasmid

#207589

Purpose

GFP-TolA deltaII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA deltaII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJS70

Plasmid

#207586

Purpose

GFP-TolA H22A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TolA H22A

Expression

Bacterial

Mutation

H22A

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pJH462

Plasmid

#172154

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (Sumo-tagged pylBCD); AraC

Expression

Bacterial

Mutation

SUMO tag fused to N-terminus of PylB

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCH1 - pBait-empty

Plasmid

#174663

Purpose

arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

Depositor

Katherine Berry

Article

Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

Type

Empty backbone

Expression

Bacterial

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJH643

Plasmid

#172168

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam

Expression

Bacterial

Mutation

sfGFP(N39*, N135*, Y151*)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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