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pFH2.30_Gag-MCP-dpol-T2A-eGFP

Plasmid

#205538

Purpose

Express HIV Gag-MCP-dpol-T2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

HIV Gag-MCP-dpol

Expression

Mammalian

Mutation

pol frameshift sequence recoded

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMH-EF1-GFP

Plasmid

#133018

Purpose

(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal GFP protein under EF1 promoter.

Depositor

Michael Huen

Article

Dong et al Proc Natl Acad Sci U S A. 2020 Jul 21;11

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Promoter

EF1

Available Since

Dec. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pJL31

Plasmid

#227428

Purpose

Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)

Depositor

Pierre Santucci

Article

Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

Insert

mWasabi

Use

CRISPR

Expression

Bacterial

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pJL32

Plasmid

#227429

Purpose

Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)

Depositor

Pierre Santucci

Article

Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:

Insert

tdTomato

Use

CRISPR

Expression

Bacterial

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFH2.101_PB-EPN11-MCP-T2A-eGFP-PGK-Blast

Plasmid

#205546

Purpose

Express EPN11-MCP-T2A-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN11-MCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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nls-Flamindo2

Plasmid

#73939

Purpose

Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

Depositor

Tetsuya Kitaguchi

Article

Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

Insert

nls-Flamindo2

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

Plasmid

#112006

Purpose

For co-expression of GCaMP6s with mKate2

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

GCaMP6s-P2A-mKate2

Use

AAV

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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IRE-SunTag

Plasmid

#196920

Purpose

The 5UTR of the ferritin heavy chain mRNA was cloned upstream of the SunTag-Renilla coding sequence in the ΔIRE-SunTag reporter

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Expression

Bacterial and Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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AL12R-HSP101

Plasmid

#161014

Purpose

PP7-tagged reporter driven by the Arabidopsis HSP101 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-Myosin

Plasmid

#79995

Purpose

LC-Myosin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

LC-Myosin (Myl9 Mouse)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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IFP2.0-C1

Plasmid

#54784

Purpose

Localization: C1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Michael Davidson, Xiaokun Shu

Article

Yu et al Nat Commun. 2014 May 15;5:3626. doi: 10.

Type

Empty backbone

Tags

IFP2.0

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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epB_UbC_tdMS2cp-tagRFP-Tx2

Plasmid

#119908

Purpose

ePiggyBac mammalian expression plasmid for tandem dimer MS2cp-tagRFP-T fusion

Depositor

Orion Weiner

Article

Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

Insert

HA-tdMS2cp-tagRFP-Tx2-nls

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Tubulin-C-18

Plasmid

#57160

Purpose

Localization: Microtubules, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Tubulin (TUBA1B Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
cr3Dd

Plasmid

#184862

Purpose

cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination system

Depositor

Patrick Sobetzko

Article

Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

Insert

Cas9, bet, exo

Tags

Cas9 ssrA degradation tag

Expression

Bacterial

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jRex-GECO

Plasmid

#169259

Purpose

AAV transfer plasmid for neuronal expression of jRex-GECO.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

JRex-GECO

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-GAL4-DBD

Plasmid

#138394

Purpose

RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4-DBD

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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JH02

Plasmid

#73511

Purpose

mCherryo_PH pCFJ150 - contians C. elegans sequence optimized mCherry

Depositor

Bob Goldstein

Article

Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

Insert

pmex-5::mCherryo::PH::tbb-2

Tags

mCherry

Promoter

pmex-5

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

Plasmid

#205760

Purpose

Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

YFP-SLBP(18-126)-IRES-H1-Skylan62A

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::ZeoR-bGHpA

Plasmid

#62441

Purpose

MXS_chaining vector with CMV::ZeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Zeocin with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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