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We narrowed to 24,097 results for: puro

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Showing: 2761 - 2780 of 24097 results

  1. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A S.pyogenes, Human)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    100936-plasmid-map-sequence-id-206124

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  2. plex_307-3xflag-ccdb-puro

    Plasmid
    #201122
    Purpose
    pLEX_307 plasmid with N-terminal FLAG tag and puro marker
    Depositor
    Alejandro Chavez
    Type
    Empty backbone
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    EF-1α
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201122-plasmid-map-sequence-id-395513

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  5. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Alexander Meissner
    Publication
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195505-plasmid-map-sequence-id-384991

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  6. qTAG-C-moxGFP-Puro-H3C2

    Plasmid
    #207783
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207783-plasmid-map-sequence-id-416185

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  7. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Robert Piper
    Publication
    Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Expression
    Mammalian
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    217971-plasmid-map-sequence-id-436106

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  8. pTS2354_Tier3(PB)-Ruby_PuroR

    Plasmid
    #169653
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a mRuby2 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mRuby2-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven mRuby2 and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    169653-plasmid-map-sequence-id-366195

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  9. MXS Chaining Kit

    Plasmid kit
    #1000000064
    Purpose
    A collection of standardized building blocks used to assemble plasmids that are optimized for fluorescence imaging or flow cytometry applications.
    Depositor
    Pierre Neveu
    Application
    Cloning and Synthetic Biology
    Vector type
    Mammalian Expression
    Cloning type
    Restriction Enzyme
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid
    #58423
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.
    Depositor
    David Ron
    Publication
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    PERK (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids M1 to Y502
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    58423-plasmid-map-sequence-id-123700

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  11. ATM-Halo HRD

    Plasmid
    #207082
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous ATM locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human ATM locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    None
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207082-plasmid-map-sequence-id-430992

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  12. RNF168-Halo HRD

    Plasmid
    #207080
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    Expression
    Mammalian
    Promoter
    None
    Available since
    May 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    207080-plasmid-map-sequence-id-432323

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  14. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  15. pGMC00009 (aka pRC0494)

    Plasmid
    #195294
    Purpose
    Lenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFP
    Depositor
    Raj Chari
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Available since
    Feb. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    195294-plasmid-map-sequence-id-385588

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  18. 3xMS-TR-100-PURO HRD

    Plasmid
    #207582
    Purpose
    homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus
    Depositor
    Jens Schmidt
    Publication
    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:
    Insert
    3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
    Tags
    3xMS2
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    207582-plasmid-map-sequence-id-429467

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  19. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Promoter
    pdx
    Available since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    67279-plasmid-map-sequence-id-115888

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  20. pCDH-EF1S-Nluc

    Plasmid
    #73033
    Purpose
    Express Nluc
    Depositor
    Kazuhiro Oka
    Insert
    Nluc
    Use
    Lentiviral and Luciferase
    Expression
    Mammalian
    Promoter
    Truncated EF1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73033-plasmid-map-sequence-id-133974

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Showing: 2761 - 2780 of 24097 results