We narrowed to 339 results for: EF1α
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Plasmid#63559PurposeExpression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Y203)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged threonine at position 203 with respect to…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Stop66)
Plasmid#63218PurposeExpression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Stop66)
UseLentiviralTagsT7 and his6ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(W66)
Plasmid#63217PurposeExpression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(W66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJM573 ZF6x6-C 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2
Plasmid#161560PurposeInducible expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the ZF6x6-C promoterDepositorInsert3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianPromoterZF6x6-CAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM576 ZF2x6-C 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2
Plasmid#161562PurposeInducible expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the ZF2x6-C promoterDepositorInsert3xFLAG-NLS-VP64-intC-ZF1-NLS-HA
UseSynthetic BiologyTags3x-FLAG and HAExpressionMammalianPromoterZF2x6-CAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-Sox2TALE-GCN4
Plasmid#120548PurposeExpress 3xNLS-Sox2 TALE-GCN4 engineered to bind a site in the human SOX2 geneDepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-NGN2TALE-GCN4
Plasmid#120547PurposeExpress 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human NGN2 eneDepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a-3NLS-Klf4 TALE-GCN4
Plasmid#120546PurposeExpress 3xNLS-Klf4 TALE-GCN4 engineered to bind a site in the human KLF4 geneDepositorAvailable SinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF_PE2
Plasmid#199267PurposeMammalian expression of SpCas9 prime editor 2 under EF1α promoterDepositorInsertPE2-P2A-mp53DD
ExpressionMammalianMutationΔ40-903Available SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAMA-YAP
Plasmid#74942PurposeLentiviral over-expression vector for YAPDepositorAvailable SinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-CDK6-mutPAM-PGK-puro
Plasmid#187638Purposesg resistant CDK6 ORFDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF5-H4K16Q-Halo-FRT
Plasmid#247458PurposeExpresses wild-type H4K16Q-Halo under EF1 promoter and can be integrated into FRT siteDepositorAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF5-H4K16R-Halo-FRT
Plasmid#247457PurposeExpresses wild-type H4K16R-Halo under EF1 promoter and can be integrated into FRT siteDepositorAvailable SinceNov. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF5-H4-HaloTag-FRT
Plasmid#247452PurposeExpresses wild-type H4-Halo under EF1 promoter and can be integrated into FRT siteDepositorAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF5-H4K16A-Halo-FRT
Plasmid#247459PurposeExpresses wild-type H4K16A-Halo under EF1 promoter and can be integrated into FRT siteDepositorAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-CuO-V5 CASC3 siRes1+2 f.l. WT
Plasmid#158540PurposeFor PiggyBac-mediated integration of V5-tagged siRNA-resistant full-length CASC3DepositorAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p27-mVenus reporter (PB)
Plasmid#190731PurposeThe p27 reporter vector, which expresses mVenus-fused, mutant (K–) p27 protein with a defective CDK binding.DepositorInsertsmVenus
p27
ExpressionBacterial and MammalianMutationp27-F62A-F64APromoterEF1αAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 2)
Plasmid#191342PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 2)
ExpressionMammalianPromoterTRE3GVAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 1)
Plasmid#191341PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 1)
ExpressionMammalianPromoterTRE3GVAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only