We narrowed to 991 results for: p16

1. pET-28a (+)-Gal4-vp16

   Plasmid

   #171075

   Purpose

   Expresses Gal4-vp16 fusion protein in bacteria

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Insert

   Gal4 DBD (NEWENTRY Budding Yeast)
   

   Use

   Tags

   His and VP16 AD (413-490)

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCVpic2neo-hTERT-p16 shRNA

   Plasmid

   #164920

   Purpose

   Expresses hTERT cDNA and p16 shRNA in mammalian cells

   Depositor

   Ji Luo

   Article

   Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

   Inserts

   human telomerase reverse transcriptase (TERT Human)
   CDKN2A (CDKN2A Human)
   

   Use

   Retroviral

   Tags

   HA tag

   Expression

   Mutation

   Promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC94-pmax-dCas9VP160-2A-puro

   Plasmid

   #48226

   Purpose

   dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
   

   Use

   CRISPR

   Tags

   2A-puro, HA Tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A;H840A (catalytically inactive)

   Promoter

   CAGGS

   Available since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-BG505.SOSIP-gp160

   Plasmid

   #123273

   Purpose

   Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (BG505) Env
   

   Use

   Tags

   CD5 leader peptide

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; SOSIP mutations

   Promoter

   CMV

   Available since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-MHL: nsp16_SARS-CoV-2

   Plasmid

   #167850

   Purpose

   Bacterial expression of the SARS-CoV-2 protein nsp16

   Depositor

   Cheryl Arrowsmith

   Article

   Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

   Insert

   nsp16
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 16, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pUAST_mLExA-VP16-NFATc1 (C-del)

   Plasmid

   #35023

   Depositor

   Jing-W Wang

   Insert

   NFATc1 (NFATC1 Human)
   

   Use

   Tags

   VP16 and mLexA

   Expression

   Insect

   Mutation

   deletion of amino acids 590-716

   Promoter

   UAS

   Available since

   March 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCG_SARS-CoV2 NSP16 C-V5-tag

   Plasmid

   #179976

   Purpose

   Mammalian expression vector for SARS-CoV-2 Nsp16, V5-tagged. Sequence codon-optimized.

   Depositor

   Konstantin Sparrer

   Article

   Hayn et al Cell Rep. 2021 May 18;35(7):109126.

   Insert

   SARS-CoV-2 Nsp16 (ORF1ab Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human codon-optimized

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

   Plasmid

   #180474

   Purpose

   Inducible gene expression vector pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

   Depositor

   Hyunju Ro

   Article

   Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

   Insert

   QFDBD-2xQFAD*-VP16*-EcR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-LacI-NLS-VP16

   Plasmid

   #103836

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   EGFP-LacI-NLS-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRcCMV Cep164 (Nigg CW325)

    Plasmid

    #41148

    Depositor

    Erich Nigg

    Article

    Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

    Insert

    Cep164 (CEP164 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCIG-VP16–RAR-alpha

    Plasmid

    #80434

    Purpose

    Expression of constitutively active human RAR-alpha in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Retinoic acid receptor alpha (RARA Human)
    

    Use

    Tags

    EGFP and VP16

    Expression

    Mammalian

    Mutation

    Promoter

    beta-actin promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

12. pLenti X2 Hygro/shp16 (w192-1)

    Plasmid

    #22264

    Depositor

    Eric Campeau

    Insert

    cyclin-dependent kinase inhibitor 2A
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. MAC-SARS-CoV-2 NSP16

    Plasmid

    #158367

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    SARS-CoV-2 NSP16 (ORF1ab )
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid

    #48227

    Purpose

    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    

    Use

    CRISPR

    Tags

    2A-neo, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti X2 Blast/shp16 (w112-1)

    Plasmid

    #22261

    Depositor

    Eric Campeau

    Insert

    sh cyclin-dependent kinase inhibitor 2A
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223 SARS-CoV-2 NSP16_nostop

    Plasmid

    #149318

    Purpose

    Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pEGFP-TetR-NLS-VP16

    Plasmid

    #103834

    Purpose

    Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    EGFP-TetR-NLS-VP16 (tetR )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV2

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-NLS-FRB-VP16

    Plasmid

    #210515

    Purpose

    expresses NLS-FRB-VP16 component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

    Insert

    NLS-FRB-VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV Lex VP16 HA (P#1708)

    Plasmid

    #14593

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA and VP16

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

20. PHR-GFP-FUSN-VP16

    Plasmid

    #183933

    Purpose

    Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

    Depositor

    Karsten Rippe

    Article

    Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

    Insert

    PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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