We narrowed to 1,074 results for: p16

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pCMV-NLS-FRB-VP16

Plasmid

#210515

Purpose

expresses NLS-FRB-VP16 component in mammalian cells

Depositor

Dongmin Lee

Article

Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

Insert

NLS-FRB-VP16

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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35S-VP16-GWY/pAM

Plasmid

#201234

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-VP16-GWY

Expression

Plant

Promoter

CaMV 35S

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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35S-GWY-VP16/pAM

Plasmid

#201232

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-VP16

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pME-GAL4-ERT2-VP16

Plasmid

#82588

Purpose

Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

GAL4-ERT2-VP16

Promoter

None

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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Split PS Intein TetR-vp16

Plasmid

#242023

Purpose

Blue-light-induced reconstitution of the tTA transactivator via split PS Intein-mediated fusion of TetR and vp16.

Depositor

Vladislav Verkhusha

Article

Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

Insert

Split PS Intein TetR-vp16

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAC154-dual-dCas9VP160-sgExpression

Plasmid

#48240

Purpose

Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9

Use

CRISPR

Tags

HA-Tag, HA-tag, and VP160

Expression

Mammalian

Mutation

D10A H840A

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pET-28a (+)-Gal4-vp16

Plasmid

#171075

Purpose

Expresses Gal4-vp16 fusion protein in bacteria

Depositor

Joan Conaway

Article

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

Gal4 DBD (NEWENTRY Budding Yeast)

Tags

His and VP16 AD (413-490)

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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ptagBFP-LacI-NLS-VP16

Plasmid

#103837

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

tagBFP-LacI-NLS-VP16

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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MSCVpic2neo-hTERT-p16 shRNA

Plasmid

#164920

Purpose

Expresses hTERT cDNA and p16 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

human telomerase reverse transcriptase (TERT Human)
CDKN2A (CDKN2A Human)

Use

Retroviral

Tags

HA tag

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP16

Plasmid

#141269

Purpose

Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway-compatible entry vector

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGTag-gal4-VP16-B-actin

Plasmid

#117817

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-TetR-NLS-VP16

Plasmid

#103834

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited tetO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-TetR-NLS-VP16 (tetR )

Expression

Mammalian

Promoter

SV2

Available Since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP Cep164 (Nigg CW324)

Plasmid

#41149

Depositor

Erich Nigg

Article

Graser et al J Cell Biol. 2007 Oct 22;179(2):321-30.

Insert

Cep164 (CEP164 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2013

Availability

Academic Institutions and Nonprofits only

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pAC94-pmax-dCas9VP160-2A-puro

Plasmid

#48226

Purpose

dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro

Use

CRISPR

Tags

2A-puro, HA Tag, and VP160

Expression

Mammalian

Mutation

D10A;H840A (catalytically inactive)

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pCIG-VP16 RARa [TJ#389]

Plasmid

#16287

Depositor

Thomas Jessell

Article

Novitch et al Neuron. 2003 Sep 25. 40(1):81-95.

Insert

RAR alpha (RARA Human)

Tags

VP16

Expression

Mammalian

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Plasmid

#180474

Purpose

Inducible gene expression vector pQFDBD-2x AD*-VP16*-EcR, α-cry:EGFP

Depositor

Hyunju Ro

Article

Hong et al Commun Biol. 2021 Dec 16;4(1):1405. doi:

Insert

QFDBD-2xQFAD*-VP16*-EcR

Use

Synthetic Biology

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pUAST_mLExA-VP16-NFATc1 (C-del)

Plasmid

#35023

Depositor

Jing-W Wang

Insert

NFATc1 (NFATC1 Human)

Tags

VP16 and mLexA

Expression

Insect

Mutation

deletion of amino acids 590-716

Promoter

UAS

Available Since

March 8, 2012

Availability

Academic Institutions and Nonprofits only

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PHR-GFP-FUSN-VP16

Plasmid

#183933

Purpose

Optogenetic PHR domain coupled to GFP, FUSN (high phase separation propensity) and VP16 activation domain; binds to CIBN upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

PHR-GFP-FUSN-VP16 (CRY2, UL48 Synthetic, Mustard Weed)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-LacI-NLS-VP16

Plasmid

#103836

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-LacI-NLS-VP16

Expression

Mammalian

Promoter

SV2

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.SOSIP-gp160

Plasmid

#123273

Purpose

Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BG505) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; SOSIP mutations

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

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